Recombinant Chicken Ubiquitin-associated domain-containing protein 1 (UBAC1)

Overview of Recombinant Chicken Ubiquitin-Associated Domain-Containing Protein 1 (UBAC1)

Recombinant Chicken Ubiquitin-Associated Domain-Containing Protein 1 (UBAC1) is a protein produced through recombinant DNA technology, utilizing chicken UBAC1 DNA sequences. UBAC1, also known as UBA Domain Containing 1, is a protein-coding gene with involvement in protein ubiquitination . It is found in the Golgi apparatus, cytosol, and plasma membrane . Recombinant UBAC1 is typically produced in host organisms like yeast to ensure it is endotoxin-free, properly folded, and post-translationally modified .

Gene and Protein Information

3.1. Role in Ubiquitination

UBAC1 is a non-catalytic component of the KPC complex, an E3 ubiquitin-protein ligase complex. This complex mediates polyubiquitination of target proteins like CDKN1B and NFKB1 .

3.2. Cell Cycle Regulation

The KPC complex, involving UBAC1, facilitates polyubiquitination and proteasome-mediated degradation of CDKN1B during the G1 phase of the cell cycle .

3.3. NF-κB Signaling

UBAC1 participates in the KPC complex, which regulates NF-κB signaling by promoting the maturation of the NFKB1 component through ubiquitination of the NFKB1 p105 precursor .

3.4. Adapter Function

Within the KPC complex, UBAC1 serves as an adapter, promoting the transfer of polyubiquitinated target proteins to the 26S proteasome .

Production and Characterization of Recombinant Chicken UBAC1

4.1. Production

Recombinant Chicken UBAC1 is often produced in yeast to ensure the protein is endotoxin-free and properly modified .

4.2. Applications

Recombinant proteins like chicken GM-CSF produced in yeast are used in immunological studies to identify suitable vaccine adjuvants .

4.3. Expression Analysis

Northern blot analysis indicates that UBAC1 expression is highest in the heart and skeletal muscle, with the lowest expression in the lung .

Immunological and Metabolic Effects

5.1. Use as an Adjuvant

Recombinant proteins are utilized alongside adjuvants to enhance vaccine efficacy. For instance, pustulan, a β-1-6-glucan, can up-regulate MHC class II expression and potentiate cytokine responses in chicken bone marrow-derived dendritic cells (BM-DCs) .

5.2. Metabolic Effects

Recombinant chicken growth hormone (rcGH) has shown significant metabolic effects in chickens, including increased plasma levels of insulin and triglycerides in female broilers .

Applications in Biotechnology

6.1. Production Platforms

Chickens can be used as bioreactors to produce recombinant human proteins in egg white. For example, human adiponectin (ADPN) has been efficiently produced in chicken egg white using a gene-targeted approach .

6.2. Large-Scale Production

Chicken bioreactor systems can provide an economical source of recombinant human proteins with proper bioactivity, useful for treating metabolic diseases .

UBAC1 Antibody Information

7.1. Antibody Details

• Antibody Name: UBAC1 antibody (19844-1-AP)

• Host: Rabbit

• Isotype: Polyclonal IgG

• Reactivity: Human, mouse, rat

• Applications: WB, IHC, ELISA

7.2. Application Details

• Western Blot (WB): 1:1000-1:4000 dilution, positive detection in mouse brain, rat liver, mouse heart, rat brain, and rat heart tissues

• Immunohistochemistry (IHC): 1:50-1:500 dilution, positive detection in human heart tissue (antigen retrieval with TE buffer pH 9.0 or citrate buffer pH 6.0 suggested)

Relevant Data Tables

Table 1: UBAC1 Expression Levels in Different Tissues

Table 2: Antibody Dilution Recommendations