Recombinant Chlamydophila psittaci Sulfur-rich protein (srp) is a protein derived from the bacterium Chlamydophila psittaci, which is known to cause psittacosis, a zoonotic disease affecting humans and birds. This protein is produced through recombinant DNA technology, allowing for its expression in host organisms like Escherichia coli. The recombinant form of the protein facilitates detailed studies on its structure, function, and potential applications in biomedical research.
Expression and Purification: The recombinant protein is typically expressed in E. coli and purified using affinity chromatography, often with a His-tag for easier purification .
Sequence and Structure: The protein consists of 160 amino acids, with a specific sequence that includes regions rich in sulfur-containing amino acids, hence its name .
Storage and Handling: It is stored in a Tris-based buffer with 50% glycerol at -20°C to maintain stability. Repeated freezing and thawing should be avoided .
| Characteristic | Description |
|---|---|
| Species | Chlamydophila psittaci (strain ATCC VR-125 / 6BC) |
| Protein Length | 160 amino acids |
| Tag | His-tag (for purification) |
| Storage Buffer | Tris-based buffer, 50% glycerol |
| Storage Conditions | -20°C, avoid repeated freezing/thawing |
| Application/Direction | Description |
|---|---|
| Biomedical Research | Study of bacterial pathogenesis and immune evasion mechanisms |
| Diagnostic Tools | Potential use in developing diagnostic assays for psittacosis |
| Vaccine Development | Investigation into its role as a potential antigen for vaccine development |
KEGG: chb:G5O_0211