Recombinant Chlorobium chlorochromatii Large-conductance mechanosensitive channel (mscL)
Description
Characteristics of Chlorobium chlorochromatii
Chlorobium chlorochromatii CaD3 is a non-motile bacterium capable of nitrogen and carbon fixation, utilizing sulfide as an electron donor for anoxygenic photosynthesis . In its symbiotic state, Chlorobium chlorochromatii CaD3 experiences limited nitrogen conditions, leading to active assimilation of ammonia via the glutamine synthetase/glutamate synthetase (GS/GOGAT) pathway . Conversely, in a free-living state, it encounters nitrogen excess and assimilates ammonia through the alanine dehydrogenase (AlaDH) pathway .
Metabolic Interactions and Symbiosis
The metabolic interactions within ‘Chlorochromatium aggregatum’ involve the exchange of metabolites between Chlorobium chlorochromatii CaD3 and the central β-proteobacterium . The chlorobia can enhance nitrogen fixation through the injection of 2-oxoglutarate from the β-proteobacterium via the periplasm . This arrangement provides motility, which is crucial for a phototrophic bacterium, along with syntrophy for nitrogen and carbon sources .
Genetic and Genomic Features
Pangenomic analysis of Chlorobium species, including C. chlorochromatii, reveals a core set of genes and gene clusters shared among different strains . These bacteria possess the potential for carbon fixation via the reductive tricarboxylic acid (rTCA) cycle, sulfur oxidation, and sulfate assimilation . They also exhibit mixotrophic capabilities, combining autotrophic and heterotrophic metabolisms .
Mechanosensitive Channels
MscL channels are known to respond to mechanical stimuli, such as changes in membrane tension . These channels play a crucial role in protecting cells from hypoosmotic shock by opening in response to increased membrane tension, allowing solutes to flow out of the cell and thereby reducing turgor pressure .
Product Specs
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Target Background
KEGG: cch:Cag_0025
STRING: 340177.Cag_0025
