Recombinant Chlorocebus aethiops Transforming growth factor beta-2 (TGFB2)

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Cat. No.
BT53054
Source
Yeast
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53054
Source
E.coli
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53054
Source
E.coli
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53054
Source
Baculovirus
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Purity
>85% (SDS-PAGE)
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Cat. No.
BT53054
Source
Mammalian cell
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Purity
>85% (SDS-PAGE)
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Description

Production and Purification

Two recombinant variants are commercially available:

Table 1: Expression Systems and Formulations

ParameterE. coli (CSB-EP023448DSU) Yeast (CSB-YP023448DSU)
Purity>85% (SDS-PAGE)>85% (SDS-PAGE)
Reconstitution0.1–1.0 mg/mL in sterile water + glycerol0.1–1.0 mg/mL in sterile water + glycerol
Storage-20°C/-80°C (lyophilized)-20°C/-80°C (lyophilized)
TagDetermined during manufacturingDetermined during manufacturing
Shelf Life12 months (lyophilized)12 months (lyophilized)

Functional and Biochemical Properties

  • Bioactivity: Recombinant TGFB2 inhibits IL-4-induced proliferation in HT-2 mouse T-cells with an ED₅₀ of 0.025–0.25 ng/mL .

  • Cross-Species Reactivity: Shares 97% amino acid identity with rodent TGFB2 and 100% identity with porcine, bovine, and canine homologs, enabling cross-species experimental use .

  • Signaling Mechanism: Binds to TGF-β receptor II (TβRII) and betaglycan (TβRIII), activating Smad-dependent and Smad-independent pathways .

Research Applications

Recombinant TGFB2 is utilized in:

  1. Cell Culture Studies: Modulates epithelial-mesenchymal transition and immune cell function .

  2. Bioassays: Quantifies activity via mink lung epithelial (Mv1Lu) cell proliferation inhibition (ED₅₀ < 40 ng/mL) .

  3. Disease Models: Investigated in fibrosis, glaucoma, and cancer due to its role in tissue remodeling .

Stability and Handling

  • Reconstitution: Requires sterile 4 mM HCl with carrier proteins (e.g., BSA) for enhanced stability .

  • Freeze-Thaw: Avoid repeated cycles; store working aliquots at 4°C for ≤7 days .

Key Citations

  1. TGFB2’s role in ocular hypertension via TRPV4 modulation .

  2. Albumin endocytosis via TβRII in endothelial cells .

  3. Structural and functional conservation across species .

Product Specs

Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins ship with standard blue ice packs. For dry ice shipping, contact us in advance; additional fees apply.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
TGFB2; Transforming growth factor beta-2 proprotein; BSC-1 cell growth inhibitor; Polyergin) [Cleaved into: Latency-associated peptide; LAP); Transforming growth factor beta-2; TGF-beta-2)]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
303-414
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
Target Names
TGFB2
Target Protein Sequence
ALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC PYLWSSDTQH SRVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGKTPKI EQLSNMIVKS CKCS
Uniprot No.
P61811

Target Background

Function
Transforming growth factor beta-2 (TGF-beta-2) is a multifunctional protein involved in processes like angiogenesis and heart development. It exists as a proprotein consisting of the Latency-associated peptide (LAP) and the active TGF-beta-2 chain. LAP keeps TGF-beta-2 inactive during storage in the extracellular matrix. LAP interacts with 'milieu molecules' like LTBP1 and LRRC32/GARP, which regulate TGF-beta-2 activation. Upon activation and release from LAP, TGF-beta-2 binds to TGF-beta receptors (TGFBR1 and TGFBR2) to transduce signals.
Protein Families
TGF-beta family
Subcellular Location
[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.

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