Recombinant Cholesterol 25-hydroxylase-like protein (CBG14675)

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Cat. No.

BT2116334

Source

in vitro E.coli expression system

Synonyms

CBG14675; Cholesterol 25-hydroxylase-like protein

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Description

Production and Purification

Recombinant CBG14675 is typically expressed in E. coli with an N-terminal His-tag for affinity purification.

Parameter	Details
Expression System	E. coli
Tag	His-tag
Purity	>90% (SDS-PAGE verified)
Storage Buffer	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	Sterile water (0.1–1.0 mg/mL) with optional glycerol for stabilization
Storage	-20°C/-80°C (long-term); 4°C (short-term aliquots)

Data aggregated from commercial suppliers .

Predicted vs. Observed Molecular Mass:

Predicted: 35.9 kDa
Observed: 31 kDa (SDS-PAGE)
Discrepancies may arise from post-translational modifications or truncations.

Stability:

Retains >95% activity after 48 hours at 37°C .
pH-sensitive; optimal activity in PBS (pH 7.4) .

Hypothesized Pathways:

Cholesterol Homeostasis: Catalyzes cholesterol oxidation to 25-hydroxycholesterol (25-HC), a regulator of lipid metabolism .
Immune Modulation: 25-HC induces chemokines like CCL5 in macrophages, linking it to inflammatory responses .

Experimental Evidence:

LPS (lipopolysaccharide) upregulates CH25H expression in mice, increasing 25-HC levels and CCL5 secretion .
C. briggsae homolog CBG14675 may share analogous roles in nematode lipid signaling, though in vivo validation is pending .

Research Applications

Recombinant CBG14675 is primarily used in structural and functional studies:

Application	Protocol Highlights	Citations
Immunogen	Generates antibodies for Western blot/ELISA
Positive Control	Validates assays targeting CH25H-like proteins
Enzyme Activity Assays	Tests catalytic efficiency in cholesterol oxidation

Limitations and Future Directions

Functional Gaps: No direct evidence of CBG14675’s enzymatic activity or in vivo role in C. briggsae.
Structural Studies: High-resolution crystallography data are lacking.
Therapeutic Potential: Mammalian CH25H is implicated in diabetic kidney disease , suggesting CBG14675 could model related pathways.

Product Specs

Form

Lyophilized powder.
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.

Lead Time

Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which may serve as a guideline.

Shelf Life

Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during the manufacturing process.
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

CBG14675; Cholesterol 25-hydroxylase-like protein

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-300

Protein Length

full length protein

Species

Caenorhabditis briggsae

Target Names

CBG14675

Target Protein Sequence

MLDLYPVHNFTVEQLEYEKNTRVLQPVWDWIKNGNEHILSSPLFPPFYALSIDYTWVAVF TFIDLFLYDVPFFKNAKIQKDRVVTWDLMKKSLKLQGWNQLLWIYPMALVQLIWVPDTEL PILAPTVFEMVSQLAIFFLAFDFTYFWFHYFNHKIKWLYRWCHSVHHMYSSPFAASAQHL HPFELFFVATFITTVPWIFPTHCLTYWLWFFVAQSVSYEVHIGYDFPFALHRIFWFYSGA PAHDMHHLRPLTCFQPWFNYLDRLMGYHITYEDLKKMTEAKFKKFGLYSVEDEKGLIKIN

Uniprot No.

Q618G2

Target Background

Function

Probable sterol desaturase.

Database Links

KEGG: cbr:CBG14675

STRING: 6238.CBG14675

Protein Families

Sterol desaturase family

Subcellular Location

Membrane; Multi-pass membrane protein.

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Recombinant Cholesterol 25-hydroxylase-like protein (CBG14675)

Description

Production and Purification

Predicted vs. Observed Molecular Mass:

Stability:

Hypothesized Pathways:

Experimental Evidence:

Research Applications

Limitations and Future Directions

Product Specs

Target Background

Quick Inquiry

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