Recombinant Chromobacterium violaceum 30S ribosomal protein S15 (rpsO)

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Description

Introduction to Recombinant Chromobacterium violaceum 30S Ribosomal Protein S15 (rpsO)

Recombinant Chromobacterium violaceum 30S ribosomal protein S15, encoded by the gene rpsO, is a crucial component of the bacterial ribosome. This protein plays a significant role in the assembly and function of the 30S ribosomal subunit, which is essential for protein synthesis in bacteria. The recombinant form of this protein is produced in yeast, offering high purity and a cost-effective option for research purposes .

Recombinant Production and Characteristics

The recombinant Chromobacterium violaceum 30S ribosomal protein S15 is produced in yeast, ensuring high purity and stability. This recombinant protein is useful for studying ribosome assembly, function, and interactions in a controlled environment. The production in yeast allows for scalable and efficient synthesis, making it accessible for various biochemical and biophysical analyses .

Research Applications

Recombinant Chromobacterium violaceum 30S ribosomal protein S15 can be used in several research applications:

  • Ribosome Assembly Studies: Understanding how S15 interacts with other ribosomal proteins and rRNA to form functional ribosomes.

  • Protein-Protein Interactions: Investigating the role of S15 in forming bridges between the 30S and 50S subunits.

  • Structural Biology: Determining the three-dimensional structure of S15 and its interactions within the ribosome.

Data and Findings

While specific data on the recombinant Chromobacterium violaceum 30S ribosomal protein S15 is limited, general findings on ribosomal protein S15 can provide insights into its function and importance:

CharacteristicsDescription
Production HostYeast
PurityHigh
FunctionEssential for 30S subunit assembly
Research ApplicationsRibosome assembly, protein-protein interactions, structural biology

References - Escherichia coli studies on ribosomal protein S15. - Recombinant Chromobacterium violaceum 30S ribosomal protein S15 product information. - General information on ribosomal proteins in prokaryotes. - Pathogenicity of Chromobacterium violaceum. - Proteomic analysis of Chromobacterium violaceum under stress conditions. - Product details for recombinant Chromobacterium violaceum 30S ribosomal protein S15.

Product Specs

Form
Lyophilized powder

Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this in your order notes, and we will fulfill your request to the best of our ability.

Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.

Note: All protein shipments default to blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.

The tag type will be determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms
rpsO; CV_1465; 30S ribosomal protein S15
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-89
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Target Names
rpsO
Target Protein Sequence
MAMTAAQKAE IVKGFQRAEG DTGSSEVQIA LLTARINDLT PHFKANTKDH HSRRGLLKLV SRRRRLLDYL KRTDAEGYRA LITRLGLRK
Uniprot No.

Target Background

Function
A crucial rRNA-binding protein, it directly interacts with 16S rRNA, facilitating the nucleation of the 30S ribosomal subunit platform by binding and bridging several 16S rRNA helices. It also forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit within the ribosome.
Database Links

KEGG: cvi:CV_1465

STRING: 243365.CV_1465

Protein Families
Universal ribosomal protein uS15 family

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