Recombinant Chromobacterium violaceum Histidine--tRNA ligase (hisS)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a guideline for your reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
hisS; CV_3537; Histidine--tRNA ligase; EC 6.1.1.21; Histidyl-tRNA synthetase; HisRS
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-423
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Target Names
hisS
Target Protein Sequence
MAQKYQAVKG MNDVLPAESY QWEYFEEALR KLLADYGYQN IRTPIVEGTP LFVRSIGEVT
DIVEKEMYTF VDSLNGDSLT LRPEGTAGTL RAVVEHNLLY NATPKLWYMG PMYRHERPQK
GRYRQFHQVG VEALGLAGPD IDAEIIAMTA DLWRRLGISQ YVRLEINSLG NAEERAAHRE
ALIAYLERHV DILDEDGKRR MHTNPLRVLD TKNPALQEMA NAAPKLSDYL GEESRAHYEG
WKAMIAALGI EYIENPRLVR GLDYYNRSVF EWVTSELGAQ GTICAGGRYD GLIEQLGGKA
ASGIGFGMGM ERVLLLLQDK GLLPAQRSVD VFLVNQGEGA GLYSMKLAQT LRAAGYSVVQ
HLGEASFKSQ MKKADGSGAE FALIVGENEI QAGQVVVKAL RADVAQQTVA ADAVLATLAT
LKA
Uniprot No.
Target Background
Database Links
KEGG: cvi:CV_3537
STRING: 243365.CV_3537
Protein Families
Class-II aminoacyl-tRNA synthetase family
Subcellular Location
Cytoplasm.
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