Recombinant Chromobacterium violaceum Protein RecA (recA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
Tag type determination occurs during production. Please specify your desired tag type for preferential development.
Synonyms
recA; CV_1607; Protein RecA; Recombinase A
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-350
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Target Names
recA
Target Protein Sequence
MASEDKSKAL AAALAQIEKQ FGKGSIMRMS DNQITENLQV ISTGSLTLDL ALGVGGLPRG
RVVEIYGPES SGKTTLCLQA VAEAQKLGGT CAYIDAENAL DPVYAQKLGV NVEDLLISQP
DTGEQALEIC DMLVRSSGVD VIVVDSVAAL VPKAEIEGEM GDSHVGLQAR LMSQALRKLT
GNIKRTNTLV IFINQIRMKI GVMFGNPETT TGGNALKFYA SVRLDIRRTG GIKKGDEVIG
NDTRVKVVKN KVSPPFRQAD FEILYGEGIS RQGEIIELGV KHGFIDKSGA WYAYNGQKIG
QGKDNTREWL KANPAIADEI ERKIREAVGV KIEINENQGD EEFADDAFDA
Uniprot No.
Target Background
Function
Recombinant Chromobacterium violaceum RecA protein catalyzes ATP hydrolysis in the presence of single-stranded DNA, facilitates ATP-dependent single-stranded DNA uptake by duplex DNA, and mediates ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA, activating it and triggering its autocatalytic cleavage.
Database Links
KEGG: cvi:CV_1607
STRING: 243365.CV_1607
Protein Families
RecA family
Subcellular Location
Cytoplasm.
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