Recombinant Citrobacter koseri UPF0442 protein CKO_03436 (CKO_03436)
Description
Introduction
Recombinant Citrobacter koseri UPF0442 protein CKO_03436 (UniProt ID: A8AM03) is a 157-amino-acid protein expressed in E. coli with an N-terminal His tag. This protein belongs to the UPF0442 family, a conserved group of bacterial proteins implicated in virulence and pathogenicity. Its recombinant form is widely used in biochemical and immunological studies to investigate C. koseri infection mechanisms and host-pathogen interactions .
Association with Virulence Mechanisms
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Iron Acquisition: CKO_03436 is linked to the High Pathogenicity Island (HPI) gene cluster in C. koseri, which facilitates iron uptake in iron-deficient host environments. This HPI shares homology with pathogenic Yersinia species and is critical for survival in mammalian hosts .
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Virulence Attenuation: Deletion of the HPI cluster (including CKO_03436 homologs) reduces C. koseri’s ability to colonize the brain and bloodstream in murine models, underscoring its role in virulence .
Functional Annotations
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Bioinformatics Insights: Comparative genomics identifies CKO_03436 as part of a core genome enriched in transport and metabolism pathways, particularly ABC transporters for amino acids and carbohydrates .
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Secretory Systems: Unlike other Citrobacter species, C. koseri lacks Type VI secretion systems (T6SS) but compensates through HPI-mediated iron scavenging .
Antibiotic Resistance Studies
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Target Identification: CKO_03436 is studied alongside other proteins (e.g., DP-3-O-acyl-N-acetylglucosamine deacetylase) to identify novel antibiotic targets, given C. koseri’s resistance to β-lactams, carbapenems, and fluoroquinolones .
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Drug Discovery: Molecular docking studies prioritize CKO_03436-interacting compounds like CMNPD13085 and CMNPD15632, which show high binding affinity in silico .
Vaccine Development
Product Specs
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Note: Proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The specific tag type is determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: cko:CKO_03436
STRING: 290338.CKO_03436
