Recombinant Citrus sinensis Pectinesterase 3

Shipped with Ice Packs
In Stock

Product Specs

Form
Lyophilized powder. We will ship the available format, but if you have specific requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional charges apply.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
Pectinesterase 3; PE 3; EC 3.1.1.11; Pectin methylesterase 3
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
267-584
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)
Target Protein Sequence
SSSV TPNVVVAADG SGNFKTVAAS VAAAPQGGTK RYIIRIKAGV YRENVEVTKK HKNIMFIGDG RTRTIITGSR NVVDGSTTFK SATVAVVGEG FLARDITFQN TAGPSKHQAV ALRVGADLSA FYNCDMLAYQ DTLYVHSNRQ FFVNCLIAGT VDFIFGNAAA VLQNCDIHAR KPNSGQKNMV TAQGRADPNQ NTGIVIQKSR IGATSDLKPV QGSFPTYLGR PWKEYSRTVI MQSSITDVIH PAGWHEWDGN FALNTLFYGE HQNAGAGAGT SGRVKWKGFR VITSATEAQA FTPGSFIAGS SWLGSTGFPF SLGL
Uniprot No.

Target Background

Function
Modifies cell walls by demethylesterifying cell wall pectin.
Protein Families
PMEI family; Pectinesterase family
Subcellular Location
Secreted, cell wall.
Tissue Specificity
In the peel, expression is localized to the region of the flavedo close to the oil glands, and to the innermost layer of the albedo. In the lamella, expression is localized to the cell layers opposing the fruit tissue, and to the parenchyma surrounding th

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