Recombinant Clostridium botulinum Cysteine desulfurase (iscS)
Shipped with Ice Packs 
In Stock 
Description
Key Features of IscS Enzymes:
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Catalytic Mechanism:
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Oxygen Resistance:
Comparative Analysis of IscS Homologs:
Recombinant Expression and Applications
While recombinant C. botulinum IscS has not been explicitly studied in the provided sources, insights can be drawn from related clostridial systems:
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Expression Systems:
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Biotechnological Potential:
Research Gaps and Future Directions
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Unresolved Questions:
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Methodological Recommendations:
Product Specs
Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them during order placement, and we will accommodate your request.
Lead Time
Delivery time varies depending on the purchasing method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipment, please contact us in advance, as additional charges will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
iscS; CBO2568; CLC_2440; Cysteine desulfurase IscS; EC 2.8.1.7
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-397
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Target Names
iscS
Target Protein Sequence
MNKQVYMDYS ATTYTKPEVL EEMLPFFTEN FGNPSSLYSF SDKTKKAVNL ARERVSKALN
AEKNEIFFTS GGSEADNWAI KGIAYANKKK GNHIITTKIE HHAILHTAQF LEKEGFKVTY
LPVDEEGFVS VEDIKNAITD ETILVSVMFA NNEIGTIEPI KEIGELCKEK NIYFHTDAVQ
AIGHVDIDVK DMNIDLLSMS AHKFYGPKGV GALYIKNGVK IQNLIHGGGQ ERGKRASTEN
TAGIVGLGKA IELAMENMPE ENEKLSNLRG RLIRGIEARI PEVKLNGPKD MSRRLPNNVN
FSFIGIEGET LLLDLDMNGI FGSTGSACAS ASLDPSHVLL SIGLPHETAH GSLRLSLGAK
NTEEDIDYVL EVLPKIIKQR REMSPLWEDY MKNKEEK
Uniprot No.
Target Background
Function
Cysteine desulfurase (iscS) is a key enzyme that provides sulfur to various partners involved in Fe-S cluster assembly, tRNA modification, and cofactor biosynthesis. It catalyzes the removal of sulfur from cysteine to produce alanine. IscS acts as a sulfur delivery protein for Fe-S cluster synthesis on IscU (an Fe-S scaffold assembly protein) and other sulfur acceptor proteins.
Database Links
KEGG: cbh:CLC_2440
Protein Families
Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
Subcellular Location
Cytoplasm.
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