Recombinant Clostridium botulinum Ribosome-recycling factor (frr)

Shipped with Ice Packs
In Stock
Cat. No.
BT48595
Source
Yeast
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT48595
Source
E.coli
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT48595
Source
E.coli
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT48595
Source
Baculovirus
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT48595
Source
Mammalian cell
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Recombinant Production and Purification

Recombinant C. botulinum frr is expressed in heterologous systems such as E. coli, yeast, or mammalian cells . Key production steps include:

ParameterDetails
Expression HostE. coli BL21, yeast, or mammalian cell lines
Purification MethodAffinity chromatography (e.g., Ni-NTA for His-tagged proteins)
Purity>95% (assessed via SDS-PAGE)
Yield~20 mg per liter of culture (for E. coli-based systems)
StorageLyophilized at -20°C in phosphate-buffered saline (PBS)

Functional Role in Ribosome Recycling

RRF collaborates with EF-G to disassemble post-termination ribosomal complexes into 30S and 50S subunits, enabling mRNA and tRNA release . Key findings include:

  • Helix H69 Displacement: RRF binding induces conformational changes in the 50S subunit’s H69 helix, destabilizing ribosomal integrity .

  • Species-Specific Activity: C. botulinum RRF functions optimally with homologous EF-G, but chimeric RRFs (e.g., E. coli Domain I + T. tengcongensis Domain II) exhibit reduced activity .

4.1. Vaccine Development

Recombinant frr is used as a subunit antigen in experimental vaccines targeting Clostridium pathogens. For example:

  • Neutralizing Antibodies: Mice immunized with recombinant C. botulinum Hc domains (a related antigen) developed protective antibodies against neurotoxins .

  • Adjuvant Compatibility: Alhydrogel® enhances frr immunogenicity, eliciting Th2-biased antibody responses .

4.2. Mechanistic Studies

  • Ribosome Dynamics: FRET-based assays and cryo-EM studies utilize recombinant frr to map ribosomal disassembly pathways .

  • Antibiotic Targets: RRF inhibitors are explored as novel antibiotics to disrupt bacterial protein synthesis .

Comparative Analysis with Other RRFs

FeatureC. botulinum RRFE. coli RRFT. tengcongensis RRF
Domain StructureTwo-domain L-shapeTwo-domain L-shapeTwo-domain L-shape
EF-G DependencyHighHighModerate
ThermostabilityModerateLowHigh
Expression Yield20 mg/L 15–30 mg/L 10–15 mg/L

Challenges and Future Directions

  • Low Solubility: Refolding protocols are required for insoluble RRF variants .

  • Species-Specificity: Engineered chimeric RRFs show reduced activity, limiting cross-species applications .

  • Therapeutic Potential: Further studies are needed to evaluate frr as a vaccine candidate against botulism .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult your local distributor for specifics. All proteins are shipped with standard blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
frr; CBO2432; CLC_2279; Ribosome-recycling factor; RRF; Ribosome-releasing factor
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-184
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)
Target Names
frr
Target Protein Sequence
MIKEILKKAD EKMGKTIVAL KKELASMKAG RANPAMLDRI EAEYYGSMTP LNQLGNISVP EARVLLIQPW DKGALSAIEK AILKSDLGLN PSNDGTVIRL VIPELTEETR KNIVKTVKKT GEEAKVAIRS IRRDCNDDVK NLKKDDVSED DIKKAEDDIQ KKTDKYIKEI DSIISAKEKE ILSI
Uniprot No.
A5I4K9

Target Background

Function
Releases ribosomes from messenger RNA at the end of protein biosynthesis. May improve translation efficiency by recycling ribosomes.
Database Links

KEGG: cbh:CLC_2279

Protein Families
RRF family
Subcellular Location
Cytoplasm.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.