Recombinant Clostridium kluyveri Translation initiation factor IF-2 (infB), partial

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Cat. No.

BT46550

Source

Yeast

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46550

Source

E.coli

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46550

Source

E.coli

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46550

Source

Baculovirus

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46550

Source

Mammalian cell

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Purity

>85% (SDS-PAGE)

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Description

Recombinant Production and Purification

Recombinant IF-2 fragments are typically expressed in E. coli systems. Key steps and properties include:

Parameter	Details
Expression system	E. coli BL21(DE3) with plasmid vectors (e.g., pET-based systems) .
Protein size	Partial IF-2 (~50–70 kDa, depending on truncation) .
Purification	Affinity chromatography (His-tag), followed by gel filtration .
Purity	>85% (verified via SDS-PAGE) .

GTPase Activity and Ribosomal Binding

IF-2’s G-domain hydrolyzes GTP upon ribosomal subunit joining. In Bacillus stearothermophilus, IF-2-G2 (isolated G-domain) retains GTPase activity and 50S subunit affinity .
Mutational studies show conserved residues (e.g., Asp in G2) are critical for GTP binding and hydrolysis .

Role in Translation Initiation

IF-2β (shorter isoform) is translated from an internal infB start site, independent of IF-2α proteolysis .
In C. kluyveri, IF-2 likely supports caproic acid biosynthesis by regulating enzymes like butyryl-CoA dehydrogenase (Bcd) and electron-transferring flavoproteins (EtfAB) .

Applications and Research Implications

Biotechnology: Engineered IF-2 variants could optimize C. kluyveri’s metabolic pathways for biofuel (e.g., hexanoate) production .
Structural studies: Recombinant IF-2 fragments enable cryo-EM and NMR studies to map ribosome interaction sites .

Comparative Genomic Analysis

C. kluyveri’s genome (strain JZZ) exhibits unique features compared to other clostridia:

Feature	C. kluyveri JZZ	C. botulinum (B2V4G9)
Chromosome length	4.45 Mb	4.1 Mb
Putative IF-2 domains	G2-G3, C1-C2	G2-G3, C1-C2
Unique genes	960 (defense mechanisms)	420 (neurotoxin synthesis)

Key Research Gaps

Direct structural or functional data on C. kluyveri IF-2 remains absent.
The interplay between IF-2 and metabolic enzymes (e.g., Bcd/EtfAB complexes) warrants experimental validation .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format, but you can specify a format when ordering, and we will fulfill your request.

Lead Time

Delivery times vary based on purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Dry ice shipping is available upon request for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize producing it with your specified tag.

Synonyms

infB; CKL_1431Translation initiation factor IF-2

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Protein Length

Partial

Purity

>85% (SDS-PAGE)

Species

Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)

Target Names

infB

Uniprot No.

A5N842

Target Background

Function

Essential for initiating protein synthesis. Protects formylmethionyl-tRNA from hydrolysis and facilitates its binding to the 30S ribosomal subunit. Also involved in GTP hydrolysis during 70S ribosomal complex formation.

Database Links

KEGG: ckl:CKL_1431

STRING: 431943.CKL_1431

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily

Subcellular Location

Cytoplasm.

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Recombinant Clostridium kluyveri Translation initiation factor IF-2 (infB), partial

Description

Recombinant Production and Purification

GTPase Activity and Ribosomal Binding

Role in Translation Initiation

Applications and Research Implications

Comparative Genomic Analysis

Key Research Gaps

Product Specs

Target Background

Quick Inquiry

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Service