Recombinant Clostridium novyi Argininosuccinate synthase (argG)

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Cat. No.
BT32623
Source
Yeast
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT32623
Source
E.coli
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT32623
Source
E.coli
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT32623
Source
Baculovirus
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT32623
Source
Mammalian cell
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Description

Molecular Characterization of argG

Argininosuccinate synthase (EC 6.3.4.5) catalyzes the penultimate step in arginine biosynthesis: the ATP-dependent condensation of citrulline and aspartate to form argininosuccinate. In C. novyi, the argG gene is part of an operon with a constitutive regulatory region, enabling continuous enzyme production even under stress conditions like hypoxia or nutrient deprivation .

PropertyDetails
Gene LocationChromosomal (part of arginine biosynthesis operon)
Enzyme FunctionCatalyzes citrulline + aspartate → argininosuccinate (ATP-dependent)
Regulatory MechanismConstitutive promoter ensures continuous expression in tumor microenvironments
Relevance in OncologySupports bacterial survival in arginine-depleted tumors

Role in Engineered C. novyi Strains

Recombinant argG is critical in oncolytic bacterial therapies, where C. novyi strains are modified to colonize tumors and lyse cancer cells. Key findings include:

  • Tumor Microenvironment Adaptation: The constitutive argG promoter ensures arginine production in nutrient-scarce tumor cores, enhancing bacterial persistence .

  • Synergy with Immune Modulation: Engineered C. novyi strains (e.g., C. novyi-NT) utilize arginine metabolism to sustain growth while secreting immune modulators (e.g., cytokines, tumor antigens) .

Tumor Localization and Immune Activation

  • Selective Colonization: C. novyi-NT spores modified with argG-driven pathways localize to hypoxic tumor regions, lysing cancer cells and releasing antigens .

  • Immune Recruitment: Arginine metabolism promotes infiltration of CD8+ T cells and macrophages, reversing immunosuppressive microenvironments .

Challenges and Innovations

  • Bacterial Clearance: Systemic administration of C. novyi-NT faces rapid phagocytic clearance, but argG-supported persistence improves tumor colonization .

  • Metabolic Engineering: Upregulating argG expression could enhance therapeutic efficacy by sustaining bacterial viability in larger tumors .

Future Directions

  • Enzyme Kinetics: Detailed studies on recombinant argG’s catalytic efficiency and stability under tumor-like conditions (pH 6.5, hypoxia) are needed.

  • Transcriptomic Optimization: Leveraging CRISPR to fine-tune argG operon expression could maximize arginine output without compromising bacterial fitness .

Product Specs

Form
Lyophilized powder. We will ship the available format, but you can specify your preference when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for details. Proteins are shipped with blue ice packs by default; request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. Specify your preferred tag type, and we will prioritize its development.
Synonyms
argG; NT01CX_0135Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-402
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Clostridium novyi (strain NT)
Target Names
argG
Target Protein Sequence
MKEKVVLAYS GGLDTSITIH WLKENYNLDV IACCVNVGQD EDFDEIKKKA IKSGATKIYV EDVKDEFVSE YIYKGVKANA VYEGKYLLGT SFARPLIAKK LVEVAHKEGA KYICHGCTGK GNDQVRFEVG IMSLDPSIKV IAPWRIWNIK SREDAVDYAN ANGIEVPVTK EKIYSRDQNL WHISHEGGDL ENIRNEHKTD MYCMTVPPEK AKDEVSYIKI TFEKGEAKKL DDVEMSPVEI LEKLNKIGGE NGIGVIDLLE NRLVGMKSRG VYETPGGTIL YAAHKELEYL TMQKETFHFK QMVSQKYGEL VYNGLWFSTL KESLDAFIDK TQEVVNGTVR LKLYKGNIMV AGMESPNALY EESISSFGAS DFYDHKDAEG FINLFGLPYK INAMIQLKNQ EN
Uniprot No.
A0Q1Z2

Target Background

Database Links

KEGG: cno:NT01CX_0135

STRING: 386415.NT01CX_0135

Protein Families
Argininosuccinate synthase family, Type 1 subfamily
Subcellular Location
Cytoplasm.

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