Recombinant Clostridium novyi Cobalamin synthase (cobS)
Description
Functional Role
CobS facilitates the condensation of adenosylcobinamide-GDP and α-ribazole-5′-phosphate to form adenosylcobalamin-5′-phosphate, a precursor to active vitamin B₁₂ . This reaction is essential for microbial cobalamin-dependent metabolism, including methionine synthesis .
Cobalamin Biosynthesis Studies
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Pathway Elucidation: CobS is used to reconstitute the nucleotide loop assembly pathway in vitro, enabling mechanistic studies of cobalamin synthesis .
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Enzyme Kinetics: Purified recombinant CobS allows precise characterization of substrate specificity and catalytic efficiency .
Vaccine Development
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Antigen Production: Recombinant CobS is a candidate for subunit vaccines targeting C. novyi infections in livestock .
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Epitope Mapping: Immunoinformatics approaches have identified CobS-derived B-cell epitopes for multi-valent vaccine design .
Industrial Biotechnology
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Cobalamin Production: Engineered E. coli expressing CobS can optimize microbial B₁₂ synthesis for industrial applications .
Comparative Functional Studies
CobS homologs across species share conserved roles in cobalamin biosynthesis but differ in membrane association and substrate handling:
Key Research Findings
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In Vitro Assembly: CobS, combined with CobU and CobT, synthesizes adenosylcobalamin-5′-phosphate from adenosylcobinamide and GTP .
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Membrane Association: CobS activity is enhanced in liposomal systems, suggesting physiological membrane dependency .
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Vaccine Potential: CobS is listed among antigens for engineered vaccines against C. novyi infections in livestock .
Future Directions
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Structural Biology: Cryo-EM or X-ray crystallography to resolve CobS’s 3D structure.
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Therapeutic Exploration: Evaluate CobS as a target for anti-clostridial drugs.
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Synthetic Biology: Optimize CobS for industrial cobalamin production via metabolic engineering.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order, and we will accommodate your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. For lyophilized form, the shelf life is 12 months at -20°C/-80°C.
Target Background
KEGG: cno:NT01CX_2079
STRING: 386415.NT01CX_2079
