Recombinant Corynebacterium urealyticum Elongation factor Ts (tsf)

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Cat. No.

BT42093

Source

Yeast

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT42093

Source

E.coli

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT42093

Source

E.coli

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT42093

Source

Baculovirus

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT42093

Source

Mammalian cell

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Description

Definition and Biological Role

Elongation Factor Ts (EF-Ts) is a guanine nucleotide exchange factor (GEF) that catalyzes the recycling of Elongation Factor Tu (EF-Tu) by displacing GDP and enabling GTP binding. This reactivates EF-Tu to form the ternary complex (EF-Tu·GTP·aa-tRNA), which delivers aminoacyl-tRNA to ribosomes during translation . In C. urealyticum, a multidrug-resistant pathogen, recombinant EF-Ts enables studies of its unique translational machinery and antibiotic resistance mechanisms .

Recombinant Production and Applications

Recombinant C. urealyticum EF-Ts is typically expressed in E. coli via plasmid vectors, purified using affinity chromatography (e.g., nickel-nitrilotriacetic acid), and validated via gel filtration . Applications include:

Antibiotic Resistance Studies: EF-Ts’ role in maintaining ternary complex stability underlies resistance to kirromycin and GE2270A-class antibiotics, which target EF-Tu .
Translational Fidelity: Pre-steady-state kinetics reveal EF-Ts accelerates ternary complex dissociation rates by 10–100×, ensuring rapid tRNA turnover .

4.1. Ternary Complex Dynamics

Formation: EF-Ts increases EF-Tu’s apparent affinity for GTP by 3.7× (K<sub>D</sub> = 12.6 nM vs. 47 nM without EF-Ts) .
Decay: EF-Ts destabilizes EF-Tu·GTP·aa-tRNA in the presence of non-hydrolyzable GTP analogs, preventing ribosomal stalling .

4.2. Antimicrobial Resistance Linkages

C. urealyticum’s EF-Ts may contribute to multidrug resistance by maintaining translation under antibiotic stress .
Rifampicin resistance in C. urealyticum involves mutations in RNA polymerase (rpoB), but EF-Ts’ role in compensating for translational errors remains unexplored .

Genomic and Evolutionary Context

Codon Usage Bias: C. urealyticum exhibits weak selected codon usage bias (S ≈ 0.45), reflecting adaptation to translational efficiency despite limited tRNA diversity .
Genomic Reduction: Its compact genome (2.37 Mb) and three rRNA operons suggest evolutionary pressure for streamlined translation machinery .

Future Directions

Structural Studies: Cryo-EM of C. urealyticum EF-Ts bound to EF-Tu could reveal species-specific adaptations.
Antibiotic Synergy: Targeting EF-Ts/Tu interactions may overcome resistance to thiazolyl peptides or kirromycin .

Product Specs

Form

Lyophilized powder. We will ship the format in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage state, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us.

Synonyms

tsf; cu0819; Elongation factor Ts; EF-Ts

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-272

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109)

Target Names

tsf

Target Protein Sequence

MANYTAADVK KLREMTGSGM MDCKKALVEA EGDFDKAIEI LRIQGAKDVG KRAERSASEG LIAVSGNTMI EVNAETDFVA KNQEFIDFAN KVAQAADAAN ANSREELEAV EVDGVKAVDA LQELSAKIGE KLELKRAVTL EGDKVAVYLH QRSADLPPAV GVLVAYEGEN EEAARAAAMQ VAALKASFLS TDDIPAETVA KEREIAEATA REEGKPEKAL PNIIEGRLKG YFKDVVLLEQ PSVTESKKTV KQVMDEAGVK LTGFVRYELG QA

Uniprot No.

B1VG90

Target Background

Function

Associates with the EF-Tu.GDP complex, promoting GDP to GTP exchange. Remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex until GTP hydrolysis on the ribosome.

Database Links

KEGG: cur:cu0819

STRING: 504474.cur_0819

Protein Families

EF-Ts family

Subcellular Location

Cytoplasm.

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Recombinant Corynebacterium urealyticum Elongation factor Ts (tsf)

Description

Definition and Biological Role

Recombinant Production and Applications

4.1. Ternary Complex Dynamics

4.2. Antimicrobial Resistance Linkages

Genomic and Evolutionary Context

Future Directions

Product Specs

Target Background

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