Recombinant Coxiella burnetii tRNA (Ile)-lysidine synthase (tilS)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, but this can be adjusted as needed.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
tilS; CBU_1509; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-449
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Target Names
tilS
Target Protein Sequence
MVELWQGLGC VFTPEKLLKF ITTLTSNSNF CIAYSGGIDS HVLVHAMSHL CQEHPWQLRA
LHINHGLNPK ANDWENHCQQ ICNRLKIPFQ SERVTLSLQP GDSIEAVARK ARYAIFQQAL
SENETLLTAH TENDQAETFL LQLLRGAGVK GLSAMPAKRK LGKGELVRPL LAITRDDLKK
YAEKNNLRWV EDDTNLELRF NRNYLRHEVL PILRRRWPEV FAVISRSANH CAEAALLLDQ
LAESDLQLIQ KDSELEILPL LQLTPERQRN VLRRWIYLHG FQLPQTKQLE QIRNDVLLAA
HDANPVFSYH TIEIRRHHGK LYLSNALSAH NATPLISWNF SRSLPLPGDL GTLIAEKKKG
VGIKTTLDTS KITVRFRQGG EQCQPAGRKE THTLKKLMQE WKIPVWQRDR VPLIYLGDKL
IAVVGYCICE GFEAKGEEWG WNVEVQPPK
Uniprot No.
Target Background
Function
This enzyme catalyzes the ATP-dependent ligation of lysine onto the cytidine at position 34 of the AUA codon-specific tRNA(Ile) containing the anticodon CAU. This cytidine-to-lysidine conversion alters the tRNA's amino acid specificity from methionine to isoleucine.
Database Links
KEGG: cbu:CBU_1509
STRING: 227377.CBU_1509
Protein Families
TRNA(Ile)-lysidine synthase family
Subcellular Location
Cytoplasm.
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