Recombinant Cricetulus griseus Galectin-1 (LGALS1)

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Cat. No.
BT55291
Source
Yeast
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55291
Source
E.coli
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55291
Source
E.coli
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55291
Source
Baculovirus
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55291
Source
Mammalian cell
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Purity
>85% (SDS-PAGE)
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Description

Production and Purification

This recombinant protein is typically expressed in E. coli, yeast, or mammalian cells (e.g., HEK293) . Purification involves affinity chromatography, with tags such as Fc or His for enhanced stability and yield . Critical quality metrics include:

  • Purity: ≥85% (SDS-PAGE)

  • Activity: Functional in glycan-binding assays, validated in apoptosis and immune modulation studies .

  • Storage: Lyophilized or liquid formats, stable at -80°C with trehalose as a cryoprotectant .

Functional Mechanisms

Recombinant C. griseus Galectin-1 operates via two primary modes:

  1. Glycan Binding: Dimerizes to crosslink LacNAc (Galβ1-4GlcNAc) residues on glycoproteins like CD4, CD45, and laminin, triggering signaling cascades .

  2. Immunomodulation:

    • Induces apoptosis in pro-inflammatory T cells (Th1/Th17) via CD7/CD45 clustering .

    • Promotes regulatory T cell (Tr1/Th2) differentiation through IL-10/IL-27 synthesis .

    • Inhibits viral entry (e.g., dengue virus) by binding viral glycans .

Table: Key Findings Using Recombinant Galectin-1

Study FocusResultSource
Cancer ImmunologySuppresses anti-tumor T cell responses; correlates with tumor aggressiveness
Viral InhibitionBlocks DENV-1 adsorption/internalization in vitro and in vivo
AutoimmunityResolves inflammation in murine models by skewing cytokine profiles
Structural AnalysisDimeric form required for LacNAc avidity and signaling

Challenges and Innovations

  • Dimerization Dependency: Functional activity requires stable homodimers, which can dissociate under low concentrations or oxidative conditions .

  • Engineering Advances: Mutagenesis (e.g., C2S substitutions) and fusion tags (Fc/His) improve stability and yield .

  • Therapeutic Potential: Targeting Galectin-1 in cancer or enhancing its activity in autoimmunity remains speculative due to pleiotropic effects .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. All proteins ship with blue ice packs by default. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
LGALS1; Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-135
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Target Names
LGALS1
Target Protein Sequence
ACGLVASNL NLKPGECLKV RGEVAPDAKS FVLNLGKDSN NLCLHFNPRF NAHGDANTIV CNSKDNGAWG TEHREPAFPF QPGSTVEVCI TFDQADLTIK LPDGHEFKFP NRLNMEAINY MAADGDFKIK CVAFE
Uniprot No.
P48538

Target Background

Function
Galectin-1 is a lectin that binds beta-galactoside and various carbohydrates. It regulates apoptosis, cell proliferation, and differentiation. It inhibits CD45 activity, preventing Lyn kinase dephosphorylation. It's a strong T-cell apoptosis inducer.
Database Links

KEGG: cge:100751963

Subcellular Location
Cytoplasm. Secreted, extracellular space, extracellular matrix. Secreted.

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