Recombinant Cricetulus griseus Phospholipase D1 (PLD1), partial

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Cat. No.
BT38782
Source
Yeast
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38782
Source
E.coli
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38782
Source
E.coli
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38782
Source
Baculovirus
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38782
Source
Mammalian cell
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Purity
>85% (SDS-PAGE)
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Description

Production and Expression Systems

Recombinant PLD1 is commonly produced in CHO cells due to their compatibility with post-translational modifications. Key steps include:

  1. Vector Construction: Cloning of the PLD1 fragment into plasmids (e.g., PB513b-1 Vector) .

  2. Transfection: Stable or transient expression in CHO-K1 or derived lines (e.g., CHO-hEPO) .

  3. Validation: Confirmation via fluorescence microscopy (e.g., GFP-tagged PLD1) and qPCR .

Table 1: Expression Metrics in CHO Systems

ParameterValue/ObservationSource
Transfection Efficiency~80% (GFP fluorescence)
PLD Activity Increase30–400-fold (vs. controls)
LocalizationEndosomes, autophagosomes

Role in Autophagy and Membrane Dynamics

  • PLD1 relocalizes to autophagosome membranes during nutrient deprivation, facilitating LC3-positive compartment expansion .

  • Inhibiting PLD1 reduces autophagosome formation by 50–80% .

Signaling Pathway Interactions

  • PKC Activation: PLD1-derived PA stimulates conventional PKC isoforms (α, β1/β2), enhancing downstream signaling .

  • mTOR Regulation: PA recruits Rheb GTPase to activate mTOR, promoting cell growth .

  • Cytoskeletal Remodeling: Phosphorylated cofilin binds PLD1, linking lipid signaling to actin dynamics .

Disease-Relevant Functions

  • Angiogenesis: PLD1 knockdown inhibits VEGF-induced endothelial cell migration and tube formation by 60–70% .

  • Cancer Survival: Overexpression suppresses p53-mediated apoptosis in DNA-damaged cells .

Applications in Biotechnology

  • Protein Production Optimization: PLD1 modulates secretory pathway efficiency in CHO cells, impacting recombinant protein yields .

  • Lipid Droplet Studies: RalA-PLD1 axis drives lipid droplet growth during nutrient stress, relevant for metabolic disease research .

Pharmacological Modulation

Inhibitor/ModulatorTargetEffect on PLD1
VU0359595PLD1-specificBlocks PA production in CHO-M1
1-ButanolSubstrate shuntReduces PA-dependent signaling
siRNA-PLD1Gene silencingDecreases LC3-II/LC3-I ratio

Table 2: PLD1 vs. PLD2 in CHO Cells

FeaturePLD1PLD2
LocalizationEndosomes, autophagosomesPlasma membrane
Activation MechanismPKC, Arf, RhoAMinimal PKC coupling
Functional RoleAutophagy, survival signalingExocytosis, membrane repair

Challenges and Future Directions

  • Partial Fragment Limitations: Truncated forms may lack full regulatory domains, altering kinetics .

  • Therapeutic Targeting: PLD1 inhibitors (e.g., ML299) show promise in cancer and angiogenesis models but require isoform specificity .

Product Specs

Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies depending on the purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with normal blue ice packs by default. For dry ice shipping, please contact us in advance; extra fees apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C is recommended. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
PLD1; Phospholipase D1; PLD 1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Target Names
PLD1
Uniprot No.
O08684

Target Background

Function
Plays a crucial role in various cellular processes, such as signal transduction, membrane trafficking, and mitotic regulation. May also regulate perinuclear intravesicular membrane traffic.
Database Links

KEGG: cge:100689404

Protein Families
Phospholipase D family
Subcellular Location
Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Late endosome membrane; Lipid-anchor; Cytoplasmic side.

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