Recombinant Cronobacter sakazakii Sulfoxide reductase heme-binding subunit YedZ (yedZ)
Description
Definition and Molecular Characterization
Recombinant Cronobacter sakazakii YedZ is a cloned version of the heme-binding subunit of sulfoxide reductase, encoded by the yedZ gene (locus: ESA_03642). This protein forms part of the YedYZ enzyme complex, which facilitates sulfoxide reduction and sulfite oxidation in gram-negative bacteria . Key features include:
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Domain architecture: Flavocytochrome with heme-binding motifs.
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Homology: Shares functional similarity with YedZ subunits in Escherichia coli and Klebsiella pneumoniae, which are implicated in sulfur metabolism .
Production and Purification
The recombinant YedZ protein is expressed in heterologous systems such as E. coli, yeast, or mammalian cells, achieving ≥85% purity via SDS-PAGE .
Applications and Research Implications
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Biochemical Studies: Used to elucidate sulfur metabolism pathways in C. sakazakii.
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Antimicrobial Targets: Potential drug target due to its role in bacterial resistance to host immune defenses .
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Industrial Enzymes: Engineered variants may aid in bioremediation of sulfite-rich environments.
Knowledge Gaps and Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will fulfill your request if possible.
Note: All protein shipments include standard blue ice packs. If dry ice shipping is required, please communicate this in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: esa:ESA_03642
STRING: 290339.ESA_03642
