Recombinant psbZ is derived from cucumber (Cucumis sativus) and replicates the full-length native protein (UniProt ID: Q4VZN6). Key specifications include:
This recombinant form retains structural fidelity to native psbZ, a transmembrane component of PSII essential for light-driven water oxidation .
Recombinant psbZ enables:
Mutagenesis assays to probe residues critical for PSII assembly.
Binding studies with extrinsic proteins (e.g., PsbQ, PsbP) to map PSII’s oxygen-evolving complex .
Stress tolerance engineering: Overexpression of related cucumber proteins (e.g., CsVI1) improves low-temperature resilience, suggesting psbZ could be a target for crop enhancement .
Structural biology: Crystallization of homologs (e.g., spinach PsbQ) provides templates for modeling psbZ’s interactions .
The cucumber psbZ shares conserved regions with homologs across species:
These variations highlight evolutionary adaptations in PSII optimization.
Ongoing research leverages recombinant psbZ to:
KEGG: csv:3429364