Recombinant Culex quinquefasciatus Eukaryotic translation initiation factor 3 subunit H (CPIJ005299)
Description
Introduction to Recombinant CPIJ005299
CPIJ005299 refers to a recombinant form of the eukaryotic translation initiation factor 3 subunit H (eIF3h) derived from Culex quinquefasciatus, a mosquito species critical as a vector for diseases such as filariasis, West Nile virus, and Zika virus . This protein is a component of the eIF3 complex, a multisubunit assembly essential for initiating translation in eukaryotes. Recombinant production of eIF3h enables researchers to study its structural, functional, and regulatory roles in mosquito biology and translation mechanisms.
Functional Role in Translation Initiation
eIF3h participates in assembling the 43S pre-initiation complex (PIC), which recruits the 40S ribosomal subunit to mRNA and facilitates scanning for the start codon . In C. quinquefasciatus, CPIJ005299 likely:
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Stabilizes eIF3 Complex Integrity: Ensures proper assembly of the eIF3 core (eIF3a, b, c) with auxiliary subunits .
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Regulates mRNA Scanning: Interacts with RNA-binding partners to enhance translation of specific mRNAs, such as viral genomes or stress-response transcripts .
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Modulates Signaling Pathways: Potentially interacts with signaling hubs like the COP9 signalosome or mTOR pathway, as observed in mammalian systems .
Vector Biology and Disease Transmission
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C. quinquefasciatus is a primary vector for filarial parasites and arboviruses. CPIJ005299 could serve as a tool to:
Resistance and Toxin Studies
While not directly linked to CPIJ005299, C. quinquefasciatus resistance to Bacillus thuringiensis toxins (e.g., Cry11Ba, Cyt1Aa) has been studied . Future work could explore whether eIF3 subunits influence toxin resistance by modulating translation of detoxification genes.
Gaps and Future Directions
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Structural Data: No high-resolution structures of CPIJ005299 or C. quinquefasciatus eIF3 complexes are available. Cryo-EM studies are needed to elucidate its role in the 43S PIC.
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Functional Specificity: Direct evidence linking CPIJ005299 to mosquito-specific translation regulation (e.g., virulence gene expression) remains limited.
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Therapeutic Potential: Exploring CPIJ005299 as a target for disrupting pathogen translation in mosquitoes could inform vector control strategies .
References
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Recombinant Culex quinquefasciatus Eukaryotic Translation Initiation Factor 3 Subunit H (CPIJ005299) – Storage guidelines and product details .
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Eukaryotic Initiation Factor 3 (eIF3) – Overview of subunit H’s role in translation and disease .
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Evolution of Resistance in Culex quinquefasciatus – Insights into toxin resistance mechanisms .
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Culex quinquefasciatus Genome – Contextualizes the species’ biological relevance .
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Reconstitution of Mammalian eIF3 – Functional core subunit interactions .
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eIF3h and Hippo Pathway – Highlights MPN domain roles in signaling .
Product Specs
Target Background
Q&A
Answer:
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Cloning and Expression: Clone the gene encoding eIF3H into an appropriate vector for expression in a suitable host system (e.g., E. coli or insect cells).
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Purification: Use affinity chromatography or other methods to purify the recombinant protein.
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Functional Assays: Conduct in vitro translation assays to assess the role of eIF3H in translation initiation. Use biochemical assays to study interactions with other eIF3 subunits or ribosomal components.
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Cellular Studies: Express the recombinant protein in mosquito cells to study its impact on cellular processes like protein synthesis and cell cycle regulation.
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Statistical Analysis: Use statistical methods (e.g., ANOVA, t-tests) to compare results from different experimental conditions.
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Replication: Repeat experiments to ensure consistency and reliability of findings.
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Literature Review: Compare results with existing literature on eIF3H function in other organisms to identify potential explanations for discrepancies.
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Alternative Assays: Employ alternative biochemical or cellular assays to validate or refute initial findings.
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CRISPR-Cas9 Gene Editing: Use CRISPR-Cas9 to knock out or knock down eIF3H in mosquito cells to study its essentiality and function.
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Proteomics: Perform mass spectrometry to identify interacting proteins and assess changes in the proteome upon eIF3H manipulation.
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RNA Interference (RNAi): Utilize RNAi to transiently reduce eIF3H expression and observe phenotypic changes in mosquitoes.
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Mosquito Strains: Ensure the use of well-characterized mosquito strains to minimize genetic variability.
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Environmental Control: Maintain consistent environmental conditions (temperature, humidity) during experiments to reduce variability.
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Bioinformatics Tools: Use bioinformatics tools to predict protein structure and function based on sequence data.
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Ethical Considerations: Ensure compliance with ethical guidelines for animal research and biosafety protocols when handling mosquitoes.
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Vector Biology: Integrate findings with studies on mosquito vector competence and disease transmission dynamics.
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Molecular Biology: Combine with research on translation initiation mechanisms in other eukaryotes to understand conserved and divergent aspects.
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Genomics and Proteomics: Use genomic and proteomic data to identify potential targets for vector control strategies.
Example Data Table: Experimental Design for Studying eIF3H Function
| Experimental Condition | Assay Type | Expected Outcome |
|---|---|---|
| eIF3H Overexpression | In Vitro Translation | Enhanced Translation Efficiency |
| eIF3H Knockdown | Cell Viability Assay | Reduced Cell Viability |
| eIF3H Mutagenesis | Protein-Protein Interaction Assay | Altered Interaction Profile |
This table illustrates how different experimental conditions can be designed to study the function of eIF3H, with expected outcomes based on the assay type.
