Recombinant Culex quinquefasciatus F-box/SPRY domain-containing protein 1 (Fsn)
Description
F-box/SPRY Domain Proteins in Biological Systems
The F-box/SPRY domain is a conserved structural motif found in proteins involved in ubiquitination pathways. For example:
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FBXO45 (F-box protein 45) in humans binds SPRY motifs in N-cadherin (NCad) to regulate neuronal migration .
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In prostate cancer, FBXO45 overexpression correlates with poor prognosis and enhanced metastatic potential .
Key functional features of F-box/SPRY proteins include:
| Feature | Role | Example |
|---|---|---|
| F-box domain | Binds Skp1 for ubiquitination | FBXO45 interaction |
| SPRY domain | Substrate recognition | NCad binding |
| Ubiquitin ligase | Targets proteins for degradation | MycBP2 interaction |
Recombinant Proteins in Culex quinquefasciatus
While recombinant proteins from Culex quinquefasciatus have been studied, the focus has been on:
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Storage proteins (e.g., LSP1 and LSP2), which accumulate during larval stages and degrade during metamorphosis .
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CWRC family proteins (Cysteine and Tryptophan-Rich), such as CqDVP-2 and CqDVP-4, which adopt β-trefoil folds for carbohydrate binding .
Critical Gaps in Current Knowledge
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No studies in the search results describe an F-box/SPRY domain-containing protein designated as "Fsn" in Culex quinquefasciatus.
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The term "Fsn" does not appear in any indexed publications related to Culex or mosquito proteomics.
Potential Hypotheses for Future Research
If "Fsn" refers to a hypothetical or uncharacterized protein, its properties might be inferred from homologous systems:
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Functional analogy: Could regulate apoptosis or neural development via SPRY-mediated interactions, similar to FBXO45 .
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Structural analogy: May require insect cell systems (e.g., Trichoplusia ni) for recombinant expression, as seen with PmFREP in shrimp .
Recommendations for Further Study
To validate the existence and role of this protein:
Product Specs
Target Background
KEGG: cqu:CpipJ_CPIJ016123
STRING: 7176.CPIJ016123-PA
