Recombinant Cyanidioschyzon merolae Pyruvate dehydrogenase E1 component subunit beta (pdhB)

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Cat. No.
BT2462280
Source
Yeast
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2462280
Source
E.coli
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2462280
Source
E.coli
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2462280
Source
Baculovirus
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2462280
Source
Mammalian cell
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Purity
>85% (SDS-PAGE)
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Description

Functional Role of pdhB in Cyanidioschyzon merolae

The pdhB gene encodes the beta subunit of the PDH complex, which is essential for cellular energy metabolism. In C. merolae, this enzyme operates under extreme environmental conditions (pH 1.5–2.5, temperatures up to 50°C), making it a model for studying thermostable metabolic machinery . The PDH complex in C. merolae likely supports mixotrophic growth, as the alga can utilize organic carbon sources like glycerol under heterotrophic conditions .

Production of Recombinant pdhB

Recombinant pdhB is generated using genetic tools tailored for C. merolae’s chloroplast or nuclear genome:

  • Chloroplast Transformation: Stable integration of exogenous genes (e.g., cat for chloramphenicol resistance) is achieved via homologous recombination . Promoters such as psbD (light-inducible) and rbcL (cell cycle-dependent) drive high-efficiency expression .

  • Nuclear Transformation: Modular plasmids enable homologous recombination into the nuclear genome, with selection markers like chloramphenicol acetyltransferase (CAT) .

Table 1: Key Parameters for Recombinant Protein Expression in C. merolae

ParameterChloroplast Expression Nuclear Expression
PromoterspsbD, rbcL, dnaKConstitutive or inducible (e.g., heat-shock)
Selection MarkerChloramphenicol (200 µg/mL)Chloramphenicol (200 µg/mL)
Doubling Time~12.7 h (under optimal light/CO₂)~1 week (heterotrophic growth)
Key ApplicationsMetabolic engineering, enzyme studiesSynthetic biology, product accumulation

Research Applications

  • Metabolic Engineering: pdhB overexpression could enhance acetyl-CoA flux for lipid or terpenoid biosynthesis .

  • Extremophile Adaptations: Study of thermostable PDH complexes informs industrial enzyme design .

  • Organelle Division: Tools like heat-shock inducible systems (e.g., CMJ101C promoter) enable conditional pdhB expression to study mitochondrial-plastid coordination .

Challenges and Future Directions

  • Expression Optimization: Despite success with chloroplast promoters, nuclear expression yields remain low .

  • Structural Studies: Cryo-EM or crystallography of C. merolae PDH complexes is needed to elucidate extremophile-specific adaptations.

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, provided as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The specific tag type is determined during production. If a particular tag type is required, please inform us for preferential development.
Synonyms
pdhB; odpB; Pyruvate dehydrogenase E1 component subunit beta; EC 1.2.4.1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-326
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Cyanidioschyzon merolae (strain 10D) (Red alga)
Target Names
pdhB
Target Protein Sequence
MLHKLFMYEA LREAIDEEMA RDKRVFVLGE DVGHYGGSYK VTKQLHTKYG DLRVLDTPIA ENSFTGMAIG AAMTGLKPVV EGMNLSFLLL AFNQISNNAG MLHYTSGGNW SIPLVIRGPG GIGKQLSAEH SQRIEAYFQA VPGLKIVACS TPYNAKGLLK AAIRDNNPVL FLEHVLLYNL KQEIPKQEYV LPLDKAQVVR EGSDVTIITY SRMLHHVMQA VKQLVAQGMN PEVIDLISLK PIDLETLVTS VSKTHKAIIV EECMQTGGIA AEVMAQIYSH AFDELDAPIR RLSSKDVPTP YNGYLEQACL VQPTQIVEAV KTLMST
Uniprot No.
Q85FX1

Target Background

Function
The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate to acetyl-CoA and CO2. This complex comprises multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3).
Database Links

KEGG: cme:CymeCp128

STRING: 45157.CMV154CT

Subcellular Location
Plastid, chloroplast.

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