Recombinant Cytochrome c1 (petC)
Description
Definition of Recombinant Cytochrome c1 (petC)
Recombinant Cytochrome c1 (petC) refers to a form of the Cytochrome c1 protein that has been produced using recombinant DNA technology . Cytochrome c1 is a subunit of the cytochrome b-c1 complex, also known as ubiquinol-cytochrome c reductase, which is an essential component of the respiratory chain in mitochondria and some bacteria .
Production of Recombinant Cytochrome c1
Recombinant Cytochrome c1 is typically produced by cloning the petC gene, which encodes for Cytochrome c1, into a suitable expression vector . This vector is then introduced into a host organism such as E. coli, yeast, baculovirus, or mammalian cells, which then produces the Cytochrome c1 protein . The recombinant protein can then be isolated and purified for use in various applications .
Function and Importance
Cytochrome c1 is a critical component of the respiratory chain, where it mediates electron transfer between cytochrome b and cytochrome c . This process is essential for generating a proton gradient across the mitochondrial membrane, which drives ATP synthesis . Research has shown that Cytochrome c1 interacts with other proteins, such as CcmI, a chaperone protein that assists in the maturation of c-type cytochromes .
Applications in Research
Recombinant Cytochrome c1 is used in various research applications, including:
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Structural and functional studies Recombinant Cytochrome c1 can be used to investigate the protein's structure, function, and interactions with other molecules .
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Drug discovery Cytochrome c1 is a potential drug target, and recombinant Cytochrome c1 can be used in screening assays to identify compounds that affect its activity .
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** изучение метаболизма лекарств** Recombinant Cytochrome c1 may be used to develop systems for the investigation of drug metabolism .
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Antibody development: Recombinant forms can assist in the development of antibodies that target Cytochrome c for research and diagnostic purposes .
Examples of Research Findings
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Studies in C. elegans Research has utilized recombinant C. elegans Cytochrome c proteins (CYC-2.1 and CYC-2.2) to understand their interactions with cardiolipin-containing liposomes, revealing structural and functional similarities to mammalian Cytochrome c .
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Maturation Studies Recombinant Cytochrome c1 has been used to study the maturation process of c-type cytochromes, including the role of chaperone proteins like CcmI .
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Mutational Analysis Mutational studies involving recombinant Cytochrome c1 have provided insights into the structure-function relationship of this protein .
Expression Systems
Product Specs
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Target Background
Recombinant Cytochrome c1 (petC) is a component of the ubiquinol-cytochrome c reductase complex (Complex III or cytochrome b-c1 complex). This complex is part of the respiratory electron transport chain, generating an electrochemical potential essential for ATP synthesis. Within this complex, Cytochrome c1 functions as an electron donor to cytochrome c.
