Recombinant D-glucuronyl C5-epimerase (hse-5)
Shipped with Ice Packs 
In Stock 
Description
Catalytic Mechanism
The enzyme operates via a proton abstraction-readdition mechanism:
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Substrate Binding: GlcA residues adjacent to N-sulfated glucosamine are positioned in the active site .
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Epimerization: Tyr residues abstract the C5 proton, inducing a conformational shift to form IdoA .
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Reversibility: Reaction equilibrium favors IdoA formation in vivo but is reversible in vitro .
Functional Insights
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Biological Role: Essential for HS chain flexibility, enabling interactions with growth factors, cytokines, and extracellular matrix proteins .
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Disease Relevance:
Enzymatic Activity Data
Comparative activity of recombinant hse-5 under varying conditions :
| Substrate | Activity (nmol/min/mg) | Optimal Conditions |
|---|---|---|
| Heparin-derived oligosaccharides | 0.8–1.2 | 30°C, 1 mM TCEP, 0.1% Tween 20 |
| K5 polysaccharide | 0.5–0.9 | pH 7.4, reducing environment required |
Activity is enhanced by reducing agents (e.g., TCEP) and detergents (e.g., Tween 20) .
Research Applications
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Chemoenzymatic Synthesis: Used to produce heparin-like glycosaminoglycans for therapeutic studies .
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Kinetic Studies: Real-time NMR and HPLC methods quantify IdoA/GlcA ratios during catalysis .
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Structural Biology: Apo- and substrate-bound crystal structures inform drug design targeting HS-related diseases .
Product Specs
Form
Lyophilized powder
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will prepare the product according to your request.
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will prepare the product according to your request.
Lead Time
Delivery time
may vary depending on the purchasing method or location. Please contact your local distributor
for specific delivery time information.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend briefly centrifuging the vial prior to opening to ensure the contents settle to the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%. Customers may use this as a reference.
Shelf Life
Shelf life is dependent on various factors including storage conditions, buffer composition, storage temperature, and the inherent stability of the protein itself.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is necessary for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during the production process. If you have a specific tag type requirement, please inform us and we will prioritize the development of the specified tag.
Synonyms
hse-5; B0285.5; D-glucuronyl C5-epimerase; Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-616
Protein Length
full length protein
Species
Caenorhabditis elegans
Target Names
hse-5
Target Protein Sequence
MKCLRWRSNRHRIYLLVACGALFLLNRHLTQEESRIDEEDEELTQVDVNEDDKKIECEPPGSIESKCIADNGKSMKCWKDEEDVYFPVSYLKKRFDMTGKLGKDGSTFELYTSYAKMRSPDSTYDPLGPFGHFSTYSVETRDRVRCVSAKTDVPMSTQWDPIPYYYPIQISQYGLQHYSRMKLDSISNKSEASPKDDVILGVNSKEWKGAAGMHETTERLFFNDEQMGKVVNISAGAALANAGAYVYLDKSPDLHVISFDWKPYEANSSFTVLAKMKQDDLLVLINYVYSEGNGKCVWQEEERISDDYIVQKPKKDGQVSYSYSYIGNSPIGEWSTVTRDLLVDVARALSSGDNRKKDDNVVLHAGDLRLVSLGFRGELTVKQKITQRREQHSHAFYAAADWLVKNQNDRGGWSVPVERSIAERKLVLPPGWHSAMAQGHGISVLTRAFKHFNDEKYLKSAAKALKLFKINSSDGGVRGEFFGNIWYEEYPTTPGSFVLNGFLYSLIGLYDLSQLELMIDENDETMRAKIQEAQELYSAGVRSLKQLLPLYDTGSGTIYDLRHVALGTAPNLARWDYHAVHVYLLKWIAGIEKDEVLSKTADRWIGYAYGKRAKHN
Uniprot No.
Target Background
Function
This enzyme catalyzes the conversion of D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acids. It plays a crucial role in the early migration of AQR and PQR neurons, which originate from the Q neuroblasts.
Gene References Into Functions
- The heparan sulfate-modifying enzyme glucuronyl C5-epimerase HSE-5 regulates Caenorhabditis elegans Q neuroblast polarization during migration. PMID: 25614236
Database Links
Protein Families
D-glucuronyl C5-epimerase family
Subcellular Location
Cell membrane; Single-pass type II membrane protein. Secreted, extracellular space, extracellular matrix, basement membrane.
Tissue Specificity
Expression in comma stage embryos is strong in the hypodermis and intestine and weaker in the head region. In late embryos, larval, and adult stages, expressed primarily in hypodermis and intestine.
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