Recombinant Danio rerio Alkaline ceramidase 1 (acer1)
Description
Biochemical Characteristics
Recombinant Danio rerio ACER1 is produced in heterologous expression systems such as E. coli and mammalian cells (e.g., HEK293) . Key features include:
The protein is typically stored in lyophilized or liquid form at -20°C/-80°C, with a shelf life of 6–12 months . Reconstitution involves dissolving in deionized water and adding glycerol (5–50%) for stability .
Functional Role in Ceramide Metabolism
ACER1 regulates sphingolipid balance by converting ceramides into sphingosine, which is further phosphorylated to sphingosine-1-phosphate (S1P) . This activity is critical for:
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Epidermal differentiation: ACER1-deficient mice exhibit transepidermal water loss and cyclic alopecia due to ceramide accumulation .
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Antimicrobial defense: Releases sphingoid bases in the stratum corneum to combat pathogens .
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Cellular stress responses: Modulates apoptosis and proliferation in keratinocytes .
Skin Homeostasis
Acer1-knockout mice showed elevated ceramide levels, disrupted hair shaft cuticles, and impaired skin barrier function, underscoring its role in epidermal integrity . ACER1 expression is upregulated during calcium-induced keratinocyte differentiation, linking it to terminal differentiation pathways .
Intestinal Barrier Function
A 2024 study identified ACER1 as a downstream target of the manganese transporter SLC39A8. Inhibition of ACER1 with D--erythro-MAPP restored sphingomyelin levels and mitigated colitis in Slc39a8-deficient mice, highlighting its role in intestinal epithelial integrity .
Comparative Analysis with Other Ceramidases
Applications in Research
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Disease modeling: Used to study skin disorders and leukodystrophy linked to ceramide dysregulation .
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Drug development: ACER1 inhibitors like D--erythro-MAPP are explored for inflammatory bowel disease and cancer .
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Enzyme kinetics: Characterized for substrate specificity toward very-long-chain ceramides .
Challenges and Future Directions
While recombinant ACER1 enables mechanistic studies, its membrane-associated nature complicates purification . Future work may focus on:
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order. We will prepare your order according to your request.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
