Recombinant Danio rerio Cytochrome c oxidase assembly protein COX14 homolog (si:dkey-222f2.8)
Description
Biological Role in Cytochrome c Oxidase Assembly
COX14 homologs are evolutionarily conserved assembly factors that stabilize newly synthesized COX1 subunits and regulate their integration into the COX complex ( ). Key mechanistic insights:
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Interaction Partners:
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Functional Impact:
3.1. Domain Architecture
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Transmembrane Topology: Single-pass mitochondrial inner membrane protein with a soluble domain facing the intermembrane space ( ).
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Conserved Motifs: Lacks cysteine residues required for disulfide bonding in mammalian COX4-2, suggesting divergent regulatory mechanisms ( ).
3.2. Evolutionary Context
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Zebrafish COX14 shares functional homology with human COX14, which coordinates copper delivery to COX2 ( ).
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Unlike mammalian COX4 isoforms, zebrafish COX14 does not exhibit hypoxia-responsive expression patterns ( ).
4.1. Experimental Use Cases
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Assembly Studies: Used to probe COX biogenesis defects in zebrafish mitochondrial disease models ( ).
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Protein-Protein Interaction Screens: Tagged variants (e.g., GFP, Myc-DDK) enable pulldown assays to identify binding partners ( ).
Critical Research Findings
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please indicate your needs during order placement. We will prepare the product according to your request.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
