Recombinant Danio rerio EF-hand calcium-binding domain-containing protein 4A (efcab4a)
Description
Human EFCAB4A (CRACR2B) and EFCAB4B (CRACR2A)
In T cells, CRACR2A forms a ternary complex with Orai1 and STIM1, dissociating upon Ca²⁺ elevation to terminate SOCE . Mutations in its EF-hand domain disrupt this regulation, leading to aberrant Ca²⁺ signaling and cell death .
Inferred Properties of Danio rerio efcab4a
While zebrafish efcab4a remains understudied, sequence homology suggests:
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Conserved domains: Two N-terminal EF-hands and a C-terminal coiled-coil motif, similar to human EFCAB4A/B .
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Functional roles: Likely modulates CRAC channels or other Ca²⁺-dependent pathways during development or immune responses.
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Experimental applications: Recombinant efcab4a could be used to study:
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Calcium dynamics in zebrafish models of disease.
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Evolutionary conservation of EF-hand protein mechanisms.
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Research Gaps and Future Directions
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Structural studies: No high-resolution structures of zebrafish efcab4a exist; comparative modeling using human homologs is needed.
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Expression profiling: Spatial and temporal expression patterns in zebrafish tissues are uncharacterized.
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Interaction mapping: Targets analogous to Orai1/STIM1 in zebrafish remain unidentified.
