Recombinant Danio rerio Melatonin receptor type 1B-A (mtnr1ba)
Description
Production and Purification
Recombinant mtnr1ba is synthesized using heterologous expression systems optimized for high yield and functional fidelity:
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Expression: Cloned into plasmid vectors under strong promoters (e.g., T7) and expressed in E. coli BL21 strains .
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Purification: Immobilized metal affinity chromatography (IMAC) leveraging the His-tag, followed by size-exclusion chromatography for monomeric isolation .
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Quality Control: Validated via Western blot, ligand-binding assays (e.g., 2-(125I)-iodomelatonin), and functional cAMP inhibition tests .
Signaling Pathways
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Melatonin Binding: Exhibits high affinity for melatonin (Kd ~0.1–1 nM), triggering Gαi-mediated inhibition of adenylate cyclase and reduced cAMP production .
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Circadian Regulation: Modulates light-dependent retinal functions and suprachiasmatic nucleus activity in zebrafish, mirroring roles observed in mammals .
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Disease Relevance: Orthologous human MTNR1B variants (e.g., p.Ala42Pro, p.Leu60Arg) disrupt receptor function and correlate with type 2 diabetes risk, suggesting conserved metabolic roles .
Experimental Applications
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Drug Discovery: Used in high-throughput screens for melatonin receptor agonists/antagonists .
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Developmental Studies: Zebrafish models exploit mtnr1ba to study embryogenesis and neurobehavioral rhythms .
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Structural Biology: Facilitates cryo-EM and X-ray crystallography studies to resolve GPCR activation mechanisms .
Comparative Analysis with Zebrafish Paralogs
Zebrafish possess duplicated melatonin receptor genes due to teleost-specific whole-genome duplication (3R event) . Key paralogs include:
Type 2 Diabetes Link
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Rare MTNR1B loss-of-function variants (e.g., p.Tyr308Ser) impair receptor signaling and elevate diabetes risk (OR = 3.88; P = 5.37×10⁻³) .
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Zebrafish mtnr1ba knockouts exhibit glucose intolerance, validating conserved metabolic roles .
Chronobiology
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Diurnal expression patterns in zebrafish brain correlate with melatonin secretion cycles, supporting evolutionary conservation of circadian regulation .
Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will accommodate your request to the best of our ability.
Note: All our proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
STRING: 7955.ENSDARP00000070419
UniGene: Dr.134330
