Recombinant Danio rerio Reactive oxygen species modulator 1 (romo1)
Description
Overview of Recombinant Danio rerio ROMO1
ROMO1 is a 79-amino acid mitochondrial inner membrane protein that regulates reactive oxygen species (ROS) production and modulates oxidative stress pathways . Recombinant versions are produced in heterologous systems like E. coli, yeast, and baculovirus for studies in oxidative stress, antibacterial activity, and mitochondrial dynamics .
Key Features
Functional Characteristics
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ROS Modulation: Induces ROS production via mitochondrial electron transport chain interactions, critical for cellular proliferation and stress responses .
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Antibacterial Activity: Disrupts bacterial membranes (e.g., S. aureus, P. aeruginosa) through membrane permeabilization .
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Mitochondrial Dynamics: Interacts with translocases (e.g., TIM16, TIM23) to regulate protein import and inner membrane integrity .
Pathway Interactions
In Zebrafish Models
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COVID-19 Cytokine Storm: Recombinant Spike protein (rSpike) upregulated romo1 mRNA, correlating with oxidative stress and inflammation. Photobiomodulation (PBM) reduced romo1 expression, suggesting therapeutic potential .
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Mitochondrial Interactions: ROMO1 partners with TIM16 (score: 0.897) and TIM23 (score: 0.832) in mitochondrial protein import .
Comparative Analysis
| Recombinant Form | Expression System | Applications | Purity |
|---|---|---|---|
| RFL29469HF | E. coli | SDS-PAGE, functional assays | >90% |
| CSB-YP020063DIL1 | Yeast | Structural studies, antibody production | Inquire |
| CSB-EP020063DIL1 | E. coli | Biotinylation assays | Inquire |
Applications in Biomedical Research
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Oxidative Stress Studies: Used to model ROS-mediated diseases (e.g., infertility, cancer) .
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Drug Screening: Evaluates compounds targeting mitochondrial ROS pathways .
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Antibacterial Development: Tests efficacy against multidrug-resistant bacteria .
Limitations and Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order notes. We will then prepare the product according to your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C, while lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
