Recombinant Danio rerio Teashirt homolog 1 (tshz1), partial

Shipped with Ice Packs
In Stock
Cat. No.
BT55582
Source
Yeast
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55582
Source
E.coli
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55582
Source
E.coli
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55582
Source
Baculovirus
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT55582
Source
Mammalian cell
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Purity
>85% (SDS-PAGE)
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Description

Developmental Roles

  • Expression Patterns:

    • Early Development: tshz1 is first detected in the anterior spinal cord at the 2-somite stage (10 hours post-fertilization) and expands posteriorly by 24 hours post-fertilization .

    • Later Stages: Expression shifts to the rhombencephalon, telencephalon, pectoral fin buds, and eyes by 48 hours post-fertilization .

    • Retinoic Acid Sensitivity: tshz1 expression is modulated by retinoic acid signaling, with exogenous retinoic acid inducing anterior neural tube expression and neckless mutants (defective in retinoic acid synthesis) showing reduced spinal cord expression .

Research Applications

This recombinant protein is utilized in:

  1. Antibody Production: To generate antibodies for immunohistochemical localization studies .

  2. Functional Assays: Investigating tshz1’s role in neural development, particularly in motor neurons and sensory organs .

  3. Protein Interaction Studies: Mapping domains involved in transcriptional complexes .

Key Research Findings

Study FocusFindingsSource
Motor Neuron DevelopmentTSHZ1 orthologs regulate survival and function of hypoglossal/phrenic motor neurons in mice; zebrafish studies suggest analogous roles .
Evolutionary ConservationTshz1’s zinc finger domains are conserved across vertebrates, supporting its role in developmental regulation .
Expression PerturbationsRetinoic acid exposure alters tshz1 expression patterns, linking it to axial patterning .

Technical Considerations

  • Sequence Limitations: The partial sequence (1–1158 aa) excludes regions critical for full-length protein activity, necessitating caution in functional extrapolation .

  • Storage Stability: Degradation occurs with repeated freeze-thaw cycles; single-use aliquots are recommended .

Future Directions

  • Functional Complementation: Testing whether zebrafish tshz1 can rescue phenotypes in Tshz1 knockout mice .

  • Structural Studies: Resolving 3D structures to elucidate DNA-binding mechanisms.

Product Specs

Form
Lyophilized powder. We will ship the in-stock format unless you specify a preference when ordering.
Lead Time
Delivery times vary by purchase method and location. Contact your local distributor for details. Proteins are shipped with blue ice packs by default; dry ice shipping is available upon request for an additional fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please let us know and we will prioritize its development.
Synonyms
tshz1; sdccag33; tsrt; Teashirt homolog 1; Teashirt-like zinc finger protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Target Names
tshz1
Uniprot No.
B3DJM5

Target Background

Function
Likely involved in developmental processes as a potential transcriptional repressor.
Gene References Into Functions
1. tsh1 (a Drosophila teashirt homologue) is expressed at the 2-somite stage in the anterior spinal cord. Expression expands throughout the spinal cord by the prim-5 stage. (PMID: 17035100)
Database Links

KEGG: dre:369196

STRING: 7955.ENSDARP00000023365

UniGene: Dr.16111

Protein Families
Teashirt C2H2-type zinc-finger protein family
Subcellular Location
Nucleus.

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