Recombinant Danio rerio UPF0249 protein ydjC homolog (ydjc)
Description
Recombinant Production and Applications
Recombinant versions of zebrafish UPF-family proteins are typically expressed in E. coli or yeast systems . For UPF0249 ydjC homolog, hypothetical production parameters include:
| Parameter | Details |
|---|---|
| Expression System | Likely E. coli (common for zebrafish proteins) |
| Tag | His-tag or Avi-tag for purification |
| Purity | >90% (via SDS-PAGE) |
| Storage | Tris/PBS buffer with glycerol, stable at -80°C |
Applications span:
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Functional studies: Investigating deacetylase roles in zebrafish development.
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Disease modeling: Insights from human YDJC in cancer migration/invasion .
Human YDJC Insights26:
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SPC-induced signaling: YDJC activates ERK, leading to keratin phosphorylation and cancer cell migration.
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Deacetylase dependency: Mutations (e.g., D13A) abolish enzymatic and signaling functions.
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Protein interactions: Binds CDC16, modulating its activity in cytoskeletal reorganization.
Zebrafish UPF-family Context35:
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UPF0197 and UPF0668: Structurally similar transmembrane proteins with roles in ion transport and development.
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Expression patterns: High in tissues requiring rapid cytoskeletal remodeling (e.g., muscle, epithelia).
Knowledge Gaps and Future Directions
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Direct functional data: No zebrafish-specific studies on UPF0249 ydjC homolog exist; existing inferences rely on human/lungfish homologs .
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Structural resolution: The full-length protein structure remains uncharacterized.
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Therapeutic potential: Deacetylase inhibitors could target YDJC-related pathways in diseases.
