Recombinant Danio rerio UPF0705 protein C11orf49 homolog (zgc:92873)

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Description

Recombinant Production

ParameterSpecification
Expression systemYeast (Saccharomyces cerevisiae)
Alternative systemsE. coli, mammalian cells, baculovirus
TagPolyhistidine (His-tag) at C-terminus
Purity>90% (SDS-PAGE/Coomassie staining)
BufferTris-based, 50% glycerol
Storage-20°C (lyophilized or aliquoted)

Expression Notes:

  • Yeast expression enables eukaryotic post-translational modifications while maintaining cost efficiency .

  • Liquid formulations have a 6-month shelf life at -20°C/-80°C; repeated freeze-thaw cycles are discouraged .

Functional and Research Applications

Primary uses:

  • ELISA development: Utilized as an antigen for antibody production due to its immunogenic epitopes .

  • Structural studies: Serves as a model for analyzing C11orf49 homologs across species .

Comparative homolog data:
While zebrafish zgc:92873 focuses on UPF0705 functionality, related isoforms in Macaca mulatta (e.g., XM_015114201.2) show splice variants with lengths ranging from 912–1,014 bp, suggesting evolutionary conservation of functional domains .

Quality Control and Validation

Analytical methods:

  • Purity assessment: SDS-PAGE with Coomassie blue staining .

  • Concentration: 0.2–2 mg/mL in Tris-glycerol buffer .

Stability considerations:

  • Lyophilized form preferred for long-term storage (>1 year at -80°C) .

  • Working aliquots stable for 1 week at 4°C .

Limitations and Future Directions

  • Species specificity: Antibodies raised against this protein may require cross-reactivity validation for human/mammalian studies .

  • Functional data gap: Current literature lacks detailed mechanistic studies on its role in zebrafish development or disease pathways.

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for fulfillment according to your requirements.
Lead Time
Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Our proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
zgc:92873UPF0705 protein C11orf49 homolog
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-331
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Target Names
zgc:92873
Target Protein Sequence
MKMNTERFNL TVDEYLAETN VLFYLNDAVT QLLEHKEEYT QFGVVRYFAE YFTSVKNGNH VLFREFSYIK ATPHNRESFI HIFWKCFRQI GKNGDLLAMS EYSSLLQLLC PDFPAEMVQN TARIVLIDDV TDCLMSFSDF IYSFQIQFFY EEFVESISAI YQDLLSGKNP NTVIVPTSTS VEQLSSSAND KPDVQEGVDA TIFCECIEGL CERFKHKYPS TVAIKEILDN TQRVSFYGFL MALAKHEGIN QDIGALPNKP DLLIDPEMDQ ELERLIAQVA ISPTSNNNSS SSALGQKEMS KKASPRKSLH QRKRIEMESD GSTEETDSSE N
Uniprot No.

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