Recombinant Debaryomyces hansenii Iron sulfur cluster assembly protein 1, mitochondrial (ISU1), partial
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchase method and location. Contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can be used as a reference.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Synonyms
ISU1; DEHA2G23958g; Iron sulfur cluster assembly protein 1; mitochondrial; Iron sulfur cluster scaffold protein 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
-
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Target Names
ISU1
Uniprot No.
Target Background
Function
Recombinant Debaryomyces hansenii Iron Sulfur Cluster Assembly Protein 1, Mitochondrial (ISU1), Partial
ISU1 serves as a mitochondrial scaffold protein crucial for the de novo synthesis of iron-sulfur (Fe-S) clusters. These clusters are essential for the maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. The process involves a two-step mechanism: (1) transient [2Fe-2S] cluster assembly on ISU1; (2) cluster transfer from ISU1 to a glutaredoxin, followed by mitochondrial [2Fe-2S] protein formation, [4Fe-4S] cluster synthesis, and targeted insertion into recipient apoproteins. This cluster assembly on ISU1 requires the cysteine desulfurase complex NFS1-ISD11 (sulfur donor), frataxin (putative iron donor), and an electron transfer chain (ferredoxin reductase and ferredoxin) that receives electrons from NADH.
ISU1 serves as a mitochondrial scaffold protein crucial for the de novo synthesis of iron-sulfur (Fe-S) clusters. These clusters are essential for the maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. The process involves a two-step mechanism: (1) transient [2Fe-2S] cluster assembly on ISU1; (2) cluster transfer from ISU1 to a glutaredoxin, followed by mitochondrial [2Fe-2S] protein formation, [4Fe-4S] cluster synthesis, and targeted insertion into recipient apoproteins. This cluster assembly on ISU1 requires the cysteine desulfurase complex NFS1-ISD11 (sulfur donor), frataxin (putative iron donor), and an electron transfer chain (ferredoxin reductase and ferredoxin) that receives electrons from NADH.
Database Links
KEGG: dha:DEHA2G23958g
Protein Families
NifU family
Subcellular Location
Mitochondrion matrix.
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