Recombinant Debaryomyces hansenii Peroxisome assembly protein 22 (PEX22)
Description
Biological Role in Peroxisome Biogenesis
PEX22 functions as a membrane anchor and co-activator for Pex4p, a ubiquitin-conjugating enzyme (E2) critical for peroxisome protein import and receptor recycling. This interaction is conserved across yeast species, though structural details vary:
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Pex4p–Pex22 Complex: In Hansenula polymorpha, Pex22p stabilizes Pex4p at the peroxisomal membrane and enhances its enzymatic activity by monoubiquitinating Pex5p (a PTS1 receptor) . While D. hansenii PEX22 shares functional homology, its exact mechanism remains under investigation.
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Peroxisomal NAD+ Homeostasis: Though PEX22 itself is not directly involved in NAD+ transport, its role in membrane protein import indirectly supports pathways like β-oxidation, which rely on NAD+ shuttles (e.g., Pmp47, Mdh3, Gpd1) .
Recombinant Production and Applications
Recombinant PEX22 is produced for structural and functional studies, leveraging E. coli expression systems. Key applications include:
Comparative Analysis with Other Species
PEX22 homologs exist in diverse organisms, with varying lengths and functional motifs:
| Organism | Length (aa) | Tag | Expression Host |
|---|---|---|---|
| Debaryomyces hansenii | 196 | His | E. coli |
| Saccharomyces cerevisiae | 180 | His | E. coli |
| Arabidopsis thaliana | 283 | His | E. coli |
Notably, D. hansenii PEX22 lacks the extended C-terminal domain seen in plant homologs, suggesting distinct evolutionary pressures .
Research Challenges and Future Directions
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Structural Elucidation: No high-resolution crystal structures for D. hansenii PEX22 exist, unlike H. polymorpha Pex22p .
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Functional Redundancy: PEX22’s role in NAD+ homeostasis is indirect, requiring further studies to clarify its interplay with Pmp47 or redox shuttles .
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Biotechnological Engineering: Efficient gene-targeting methods in D. hansenii (e.g., PCR-based homologous recombination) could enable PEX22 overexpression for industrial applications .
Product Specs
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Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If a specific tag is required, please inform us for preferential development.
Target Background
KEGG: dha:DEHA2F01386g
