Recombinant Dechloromonas aromatica Disulfide bond formation protein B (dsbB)
Description
Overview of Disulfide Bond Formation Protein B (DsbB)
DsbB is a membrane-bound oxidoreductase critical for catalyzing disulfide bond formation in bacterial periplasmic and secreted proteins. In Escherichia coli, DsbB regenerates the active-site disulfide of DsbA, the primary oxidase responsible for introducing disulfide bonds into substrate proteins . This process involves electron transfer from reduced DsbA to ubiquinone/menaquinone via DsbB’s two catalytic disulfide pairs (Cys41–Cys44 and Cys104–Cys130) . While DsbB homologs are well-characterized in model organisms like E. coli and Salmonella, limited data exist for Dechloromonas aromatica, a soil microbe known for anaerobic benzene degradation and perchlorate reduction .
Genomic and Functional Insights into Dechloromonas aromatica DsbB
Recombinant Expression and Applications
Recombinant D. aromatica DsbB has not been explicitly studied, but heterologous expression systems (e.g., E. coli) are commonly used to produce disulfide bond-forming proteins . Challenges include:
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Membrane Localization: DsbB’s integral membrane nature complicates soluble expression .
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Redox Activity: Requires co-expression with partner oxidase (e.g., DsbA) and quinones .
Potential Applications:
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Bioremediation: Enhancing oxidative folding of enzymes involved in pollutant degradation .
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Industrial Protein Production: Optimizing disulfide bond formation in recombinant proteins .
Research Gaps and Future Directions
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Structural Characterization: No crystal structures or biochemical assays for D. aromatica DsbB exist. Comparative studies with E. coli DsbB (PDB: 1FVK) could clarify evolutionary adaptations .
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Redox Potential: Measurement of its catalytic disulfide bonds’ redox potential (e.g., via cyclic voltammetry) is needed .
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Substrate Specificity: Identification of partner oxidases (e.g., DsbA homologs) in D. aromatica .
Comparative Analysis with Model Systems
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional charges will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: dar:Daro_3145
STRING: 159087.Daro_3145
