Recombinant Dehalococcoides sp. Nucleoside diphosphate kinase (ndk)

Shipped with Ice Packs
In Stock
Cat. No.
BT42881
Source
Yeast
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42881
Source
E.coli
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42881
Source
E.coli
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42881
Source
Baculovirus
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42881
Source
Mammalian cell
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Functional Role of NDK in Microbial Systems

NDK catalyzes the reversible transfer of γ-phosphate between nucleoside triphosphates (NTPs) and diphosphates (NDPs), maintaining cellular nucleotide pools critical for DNA/RNA synthesis and energy metabolism . In Dehalococcoides, this enzyme may support metabolic processes linked to reductive dechlorination, though direct evidence remains limited. Structural studies of NDK homologs (e.g., in Aspergillus flavus and plants) reveal conserved ferredoxin-like folds and catalytic residues (Arg-104, His-117, Asp-120) essential for phosphotransferase activity .

Recombinant NDK Production and Applications

Recombinant NDK is typically generated via heterologous expression in E. coli, followed by purification (e.g., affinity chromatography) . Although Dehalococcoides-specific NDK expression systems are not described in the provided sources, analogous workflows apply:

  • Enzyme Kinetics:

    • Recombinant NDK from Aspergillus flavus shows VmaxV_{\text{max}} of 12.4 μmol/min/mg and KmK_m of 0.8 mM for ATP .

    • Inhibitors like azidothymidine (AZT) suppress spore formation in fungi, hinting at NDK’s regulatory roles .

Research Gaps and Future Directions

  • Potential Roles in *Dehalococcoides*:

    • Supporting nucleotide metabolism during energy-limited organohalide respiration.

    • Interacting with RDases or nitrogenase complexes, given Dehalococcoides’ reliance on hydrogen and acetate .

  • Experimental Priorities:

    • Cloning and characterization of Dehalococcoides NDK genes.

    • Structural analysis to identify unique active-site adaptations.

Implications for Bioremediation

Dehalococcoides strains are pivotal in bioremediation due to their ability to detoxify chlorinated ethenes . While NDK’s role here is speculative, its manipulation could enhance metabolic resilience. For instance, NDK inhibitors (e.g., flavonoids) might modulate microbial activity in contaminated sites .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize development of that tag.
Synonyms
ndk; DehaBAV1_0373; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-142
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Target Names
ndk
Target Protein Sequence
MERTLLLVKP DGVNRGLSGE ILGRMEKLGL KLIGLRMLQM DAVLADKHYA PHRARPFFKD LVTYITSGPI TAAVFEGENA VEKMRKAMGA TDPAKSEKGT VRGDLGIDIE QNTVHGSDSA ENAKHEISLF FSESELVNYD RR
Uniprot No.
A5FS65

Target Background

Function
Plays a major role in synthesizing nucleoside triphosphates (other than ATP). Transfers the ATP gamma phosphate to the NDP beta phosphate via a ping-pong mechanism using a phosphorylated active-site intermediate.
Database Links

KEGG: deb:DehaBAV1_0373

Protein Families
NDK family
Subcellular Location
Cytoplasm.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.