Recombinant Dehalococcoides sp. Translation initiation factor IF-2 (infB), partial
Description
Biological Context of Translation Initiation Factor IF-2
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Role in Translation Initiation:
IF-2 is essential for recruiting the initiator tRNA (fMet-tRNA) to the ribosomal P-site and stabilizing the 30S–50S ribosomal subunit association during translation initiation . -
Genomic and Structural Features:
The infB gene in Dehalococcoides sp. likely encodes multiple IF-2 isoforms, as observed in other bacteria. Structural studies reveal that IF-2 domains (e.g., G2 and C-terminal regions) exhibit mobility, enabling conformational changes critical for function .
Functional Insights from Homologous Systems
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GTPase Activity:
IF-2 hydrolyzes GTP to promote ribosomal subunit joining and transition to elongation . Mutations in IF-2’s GTP-binding domain impair translation fidelity . -
Genome Integrity:
Full-length IF-2 isoforms in E. coli influence replication restart mechanisms during DNA damage, suggesting a role beyond translation .
Implications for Dehalococcoides Physiology
Dehalococcoides spp. are obligate organohalide-respiring bacteria critical for bioremediation . Their reliance on hydrogen as an electron donor and reductive dehalogenases (e.g., RdhA) for energy metabolism suggests tight coupling between translation efficiency and catabolic activity. Recombinant IF-2 studies could elucidate:
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Adaptation to stress during organohalide respiration.
Research Gaps and Future Directions
Product Specs
Target Background
KEGG: deb:DehaBAV1_0874
