Recombinant Deinococcus proteolyticus Chaperone protein DnaK (dnaK), partial

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Cat. No.

BT33365

Source

Yeast

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33365

Source

E.coli

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33365

Source

E.coli

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33365

Source

Baculovirus

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33365

Source

Mammalian cell

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Purity

>85% (SDS-PAGE)

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Description

General Overview of DnaK in Deinococcus Species

DnaK is a member of the Hsp70 heat shock protein family, functioning as a molecular chaperone that assists in protein folding, prevents aggregation under stress, and participates in DNA repair processes. In Deinococcus proteolyticus, this protein is critical for survival under extreme conditions, such as oxidative stress and UV radiation, which aligns with the genus’s renowned resilience .

Key Features of DnaK:

Domain Structure:
- N-terminal ATPase domain (binds ATP)
- Substrate-binding domain (interacts with client proteins)
- C-terminal lid region (regulates substrate release)
Role in Stress Response:
Facilitates renaturation of denatured proteins during environmental stress .

Recombinant DnaK Production

While no studies explicitly describe the recombinant production of D. proteolyticus DnaK, protocols for homologous proteins in D. radiodurans and other extremophiles provide a framework:

Typical Cloning and Expression Workflow:

Step	Description	Host System	Reference
Gene Amplification	Partial dnaK gene cloned from D. proteolyticus genomic DNA	PCR-based	—
Vector Construction	Insertion into pET or pGEX vectors for expression	E. coli BL21(DE3)
Protein Purification	Affinity chromatography (e.g., His-tag) followed by size-exclusion chromatography	—	—

Functional Insights from Homologs

Comparative genomic analysis between D. proteolyticus and D. radiodurans reveals conserved stress-response mechanisms :

Shared Stress-Response Pathways:

Pathway	Key Proteins	Role in Deinococcus
Protein Folding	DnaK, GroEL/ES	Mitigates oxidative damage to proteome
DNA Repair	RecA, RecFOR	Restores genome integrity post-irradiation

D. proteolyticus exhibits enhanced oxidative stress resistance compared to D. radiodurans, suggesting potential differences in chaperone activity or regulation .

Research Gaps and Future Directions

No peer-reviewed studies directly characterize recombinant D. proteolyticus DnaK. Critical unanswered questions include:

Structural Specificity: How does the partial sequence affect ATPase or substrate-binding activity?
Functional Redundancy: Does DnaK collaborate with other chaperones (e.g., DdrB) under stress?
Biotechnological Applications: Potential use in industrial protein refolding systems.

Comparative Genomic Data

Analysis of D. proteolyticus versus D. radiodurans genomes :

Feature	D. proteolyticus	D. radiodurans
Genome Size	~3.1 Mb	~3.3 Mb
GC Content	65.6%	67%
Core Genes (COG Categories)	1,489 (65% metabolism)	1,489 (63% metabolism)
Stress-Response Genes	118 unique	132 unique

Product Specs

Form

Lyophilized powder. We will ship the available format, but you can request a specific format when ordering.

Lead Time

Delivery time varies by purchase method and location. Consult local distributors for details. Proteins are shipped with blue ice packs by default; dry ice shipping is available upon request for an extra fee.

Notes

Avoid repeated freezing and thawing. Working aliquots are stable at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

dnaK; Deipr_1906Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Protein Length

Partial

Purity

>85% (SDS-PAGE)

Species

Deinococcus proteolyticus (strain ATCC 35074 / DSM 20540 / JCM 6276 / NBRC 101906 / NCIMB 13154 / VKM Ac-1939 / CCM 2703 / MRP)

Target Names

dnaK

Uniprot No.

P94695

Target Background

Function

Acts as a chaperone.

Database Links

KEGG: dpt:Deipr_1906

STRING: 693977.Deipr_1906

Protein Families

Heat shock protein 70 family

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Recombinant Deinococcus proteolyticus Chaperone protein DnaK (dnaK), partial

Description

General Overview of DnaK in Deinococcus Species

Key Features of DnaK:

Recombinant DnaK Production

Typical Cloning and Expression Workflow:

Functional Insights from Homologs

Shared Stress-Response Pathways:

Research Gaps and Future Directions

Comparative Genomic Data

Product Specs

Target Background

Quick Inquiry

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