Recombinant Desulfotalea psychrophila 50S ribosomal protein L22 (rplV)

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Cat. No.
BT2434853
Source
Yeast
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434853
Source
E.coli
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434853
Source
E.coli
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434853
Source
Baculovirus
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434853
Source
Mammalian cell
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Purity
>85% (SDS-PAGE)
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Description

Introduction to Recombinant Desulfotalea psychrophila 50S Ribosomal Protein L22 (rplV)

Recombinant Desulfotalea psychrophila 50S ribosomal protein L22 (rplV) is a genetically engineered protein derived from the psychrophilic (cold-loving) sulfate-reducing bacterium Desulfotalea psychrophila. This protein belongs to the universal ribosomal protein family uL22 and plays a critical role in the assembly and function of the 50S ribosomal subunit, which is essential for bacterial protein synthesis .

Genomic and Proteomic Context

FeatureDetailSource
Genome OrganizationrplV is located on the circular chromosome of D. psychrophila LSv54.
Plasmid AssociationsNo direct plasmid linkage reported; rplV is part of core ribosomal genes.
Cold Shock RegulationOverexpression of L22 observed in Enterococcus faecalis ΔcspR mutants, suggesting post-transcriptional regulation .

Research Findings and Applications

Antibiotic Resistance

  • Mutations in rplV (e.g., premature stop codons) have been identified in methicillin-resistant Staphylococcus aureus (MRSA) strains, potentially affecting ribosomal function and antibiotic susceptibility .

  • Structural studies in Streptococcus pneumoniae and Anaplasma phagocytophilum reveal L22’s role in stabilizing 23S rRNA domains targeted by macrolides (e.g., erythromycin) .

Biotechnological Relevance

  • Recombinant Production:

    • Expressed in E. coli with >85% purity (SDS-PAGE), often with tags for purification .

    • Buffer: Tris/PBS-based, 6% trehalose; storage at -20°C/-80°C .

  • Potential Uses:

    • Structural studies of ribosomal dynamics.

    • Development of cold-adapted biocatalysts for industrial processes .

Comparative Analysis of L22 Across Organisms

OrganismKey FeaturesSource
Desulfotalea psychrophilaCold-adapted; core ribosomal gene; limited direct studies.
Streptococcus pneumoniaeBinds 23S rRNA; interacts with L4, L17, L20; critical for 50S assembly.
Anaplasma phagocytophilumFull-length recombinant protein (1–112 aa); >90% purity.
Bacillus subtilisInteracts with RqcH during ribosome quality control; marks stalled nascent chains.

Gaps and Future Directions

  • Limited Direct Studies: Most data derive from homologs (e.g., S. pneumoniae, A. phagocytophilum).

  • Cold-Adaptation Mechanisms: No experimental validation of D. psychrophila L22’s structural adaptations to low temperatures.

  • Regulatory Networks: Potential interactions with cold shock proteins (e.g., CspR) remain unexplored .

Product Specs

Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized fulfillment.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All protein shipments include standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Synonyms
rplV; DP1129; 50S ribosomal protein L22
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-110
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Desulfotalea psychrophila (strain LSv54 / DSM 12343)
Target Names
rplV
Target Protein Sequence
MEARAVGKYI RISPQKARLV ADVVRGMGVD QAITTLRFMP KKGAVILQKV IESALANATQ DDQADVDNLY VKVITIDGGP SLKRIRPRAM GRATGIIKRT SHITVVLDEN
Uniprot No.
Q6AP66

Target Background

Function
This protein exhibits specific binding to 23S rRNA, a process enhanced by other ribosomal proteins such as L4, L17, and L20. It plays a crucial role in the early stages of 50S ribosomal subunit assembly. The protein interacts with multiple domains of the 23S rRNA within both the assembled 50S subunit and the complete 70S ribosome. Its globular domain is located near the polypeptide exit tunnel on the subunit's exterior, while an extended beta-hairpin structure lines the exit tunnel wall within the 70S ribosome.
Database Links

KEGG: dps:DP1129

STRING: 177439.DP1129

Protein Families
Universal ribosomal protein uL22 family

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