Recombinant Desulfovibrio vulgaris Tyrosine--tRNA ligase (tyrS)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for custom preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a reference.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Synonyms
tyrS; DVU_0953; Tyrosine--tRNA ligase; EC 6.1.1.1; Tyrosyl-tRNA synthetase; TyrRS
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-398
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
Target Names
tyrS
Target Protein Sequence
MIDIDRQLEH IKRGCAELID EGELRKKLER GTPLRIKAGF DPTAPDLHLG HTVLIHKLRH
FQELGHTVIF LIGDFTGLIG DPSGRSDTRP PLTREQVLAN AETYKQQVFK ILDPEKTVVD
FNSRWMGEFG AADFIRLASR YTVARMMERD DFEKRYKEGR PIAVHEFLYP LVQGYDSVAL
KADVELGGTD QKFNLLVGRH LQSQYGQEPQ CILTMPLLEG LDGVKKMSKS LGNYVGIDES
PADMFGKLMS VSDELMWRYF ELISSRSLDE IADLRRKVET GEAHPKLVKE SLAYELTTRY
HGEDKAAEAQ QGFNAVFAGG GVPDDAPVHA CDHGDDSTPP AFLEAAGLVK SRGEAKRLIK
EGALSVDGVR CDDANSPLAS GEYVIKLGKK RFLRLTVR
Uniprot No.
Target Background
Function
This enzyme catalyzes the attachment of tyrosine to tRNA(Tyr) through a two-step reaction. First, tyrosine is activated by ATP to form Tyr-AMP. This activated tyrosine is then transferred to the acceptor end of tRNA(Tyr).
Database Links
KEGG: dvu:DVU0953
STRING: 882.DVU0953
Protein Families
Class-I aminoacyl-tRNA synthetase family, TyrS type 2 subfamily
Subcellular Location
Cytoplasm.
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