Recombinant Dictyostelium discoideum 60S ribosomal protein L3 (rpl3)
Description
Functional Roles in Ribosomal Activity
RPL3 is indispensable for ribosomal function:
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Peptidyltransferase Center (PTC) Formation: RPL3’s central extension anchors rRNA helices (e.g., helix 90–92) near the PTC, directly influencing catalytic activity .
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Aminoacyl-tRNA Binding: Mutations in RPL3 (e.g., W255C) alter aa-tRNA accommodation rates and confer antibiotic resistance (e.g., anisomycin) .
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Interactions with Signaling Pathways: In Dictyostelium, RPL3 binds Glycogen Synthase Kinase-3 (GSK-3), suggesting roles in chemotaxis, cytokinesis, and developmental regulation .
Functional Studies in Dictyostelium
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GSK-3 Interaction: Affinity chromatography identified RPL3 as a binding partner of GSK-3 in Dictyostelium, implicating it in cytoskeletal regulation and developmental signaling .
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Developmental Defects: Dictyostelium RPL3 knockout cells exhibit impaired chemotaxis, delayed cytokinesis, and reduced cell size under stress .
Disease Associations
While Dictyostelium RPL3 itself is not directly linked to human diseases, its human homolog is associated with:
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Diamond-Blackfan Anemia: Mutations in RPL3 disrupt erythroid differentiation .
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Viral Interactions: Binds HIV-1 TAR mRNA, potentially modulating viral replication .
Applications of Recombinant RPL3
Product Specs
Target Background
KEGG: ddi:DDB_G0291862
STRING: 44689.DDB0191094
