Recombinant Dictyostelium discoideum Metabotropic glutamate receptor-like protein O (grlO)
Description
Overview and Classification
Recombinant Dictyostelium discoideum Metabotropic Glutamate Receptor-Like Protein O (GrlO) is a member of the Family 3 G-protein-coupled receptors (GPCRs) encoded by the grlO gene. This family comprises 17 receptors (GrlA–GrlR) in D. discoideum, which share structural and functional similarities with animal metabotropic glutamate receptors (mGluRs) and γ-aminobutyric acid B (GABA<sub>B</sub>) receptors . GrlO is classified as G-protein-coupled receptor family 3 protein 14 and is implicated in sensing extracellular ligands to regulate developmental and environmental responses .
Recombinant Production
GrlO is produced recombinantly using heterologous expression systems:
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Expression Systems: Cell-free expression, E. coli, yeast, or mammalian cells .
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Purification: Affinity chromatography (e.g., Ni-NTA for His-tagged variants) .
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Functional Assays: Ligand-binding studies and G-protein activation assays (methods inferred from related Grl proteins) .
Research Gaps and Future Directions
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Ligand Specificity: The endogenous ligand(s) for GrlO remain unidentified.
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Knockout Phenotypes: No published studies describe grlO<sup>−</sup> mutants.
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Cross-Species Homology: GrlO lacks direct orthologs in vertebrates, limiting comparative analyses .
Table 2: Grl Family Members in D. discoideum
Applications in Research
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Signal Transduction Studies: Tools for dissecting GPCR signaling in a genetically tractable model .
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Evolutionary Biology: Insights into the origin of Family 3 GPCRs predating animal-fungal divergence .
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Drug Discovery: Screening platform for novel GPCR ligands (given structural conservation with human receptors) .
References
Product Specs
Please note: We prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order notes, and we will accommodate your request.
All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance, as additional fees will apply.
Generally, liquid form has a 6-month shelf life at -20°C/-80°C. Lyophilized form has a 12-month shelf life at -20°C/-80°C.
Target Background
KEGG: ddi:DDB_G0271688
