Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0287489 (DDB_G0287489)
Description
Introduction to Recombinant Dictyostelium discoideum Putative Uncharacterized Protein DDB_G0287489 (DDB_G0287489)
Recombinant DDB_G0287489 is a bioengineered version of a hypothetical protein encoded by the DDB_G0287489 gene in Dictyostelium discoideum, a model organism for studying cellular processes such as phagocytosis, differentiation, and host-pathogen interactions . While its biological function remains uncharacterized, its recombinant form is commercially available for research purposes, enabling structural and functional studies. Below, we synthesize available data on its characteristics, production, and potential applications.
Recombinant Expression System
The protein is expressed in E. coli with an N-terminal His-tag for purification. Key production details include:
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Form: Lyophilized powder in Tris/PBS buffer with 6% trehalose, pH 8.0 .
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Reconstitution: Recommended in deionized sterile water (0.1–1.0 mg/mL), with optional glycerol (5–50%) for stabilization .
Current Knowledge Gaps
Despite its availability, DDB_G0287489 remains functionally uncharacterized. Limited data suggest:
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Hypothetical Role: Belongs to a family of uncharacterized proteins in D. discoideum, potentially involved in cellular processes conserved in eukaryotes .
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Lack of Functional Studies: No direct evidence links DDB_G0287489 to bacteriolytic activity, phagocytosis, or development, unlike other D. discoideum proteins (e.g., BadA, Kil1) .
Potential Research Directions
| Area of Study | Rationale |
|---|---|
| Structural Analysis | Crystallization or NMR studies to resolve tertiary structure |
| Functional Screening | Yeast two-hybrid or co-IP assays to identify binding partners |
| Knockout/Knock-in Models | Genetic manipulation in D. discoideum to assess phenotypic effects |
DDB_G0287489 vs. Bacteriolytic Proteins in D. discoideum
| Protein | Function/Activity | Bacteriolytic Role | Source |
|---|---|---|---|
| DDB_G0287489 | Uncharacterized | N/A | |
| BadA | Bacteriolytic activity against K. pneumoniae | Confirmed | |
| Kil1 | Critical for bacterial killing in phagosomes | Confirmed |
Challenges and Future Outlook
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please indicate them in your order. We will fulfill your request whenever possible.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. For shipments with dry ice, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid forms is 6 months at -20°C/-80°C, while lyophilized forms can be stored for 12 months at -20°C/-80°C.
Target Background
KEGG: ddi:DDB_G0287489
