Recombinant Dictyostelium discoideum Putative uncharacterized transmembrane protein DDB_G0285385 (DDB_G0285385)
Description
Protein Overview
DDB_G0285385 is encoded by the gene DDB_G0285385 in Dictyostelium discoideum. It is annotated as a putative transmembrane protein with a length of 303 amino acids (UniProt ID: Q54N94) . Recombinant versions are produced for research applications, enabling biochemical and structural studies.
Key Attributes:
Recombinant Production
The recombinant protein is expressed in E. coli and purified using affinity chromatography. Key steps include:
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Construct Design: Codon-optimized for prokaryotic expression.
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Purification: His-tag affinity followed by size-exclusion chromatography .
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Storage: Lyophilized powder in Tris/PBS buffer with 6% trehalose (pH 8.0), stable at -20°C/-80°C .
Expression and Quality Control:
| Parameter | Details |
|---|---|
| Purity | >90% (SDS-PAGE verified) |
| Reconstitution | Deionized water or custom buffers (e.g., with glycerol for stability) |
| Applications | ELISA, structural studies, interaction assays |
Functional Insights
While the exact role of DDB_G0285385 is unknown, its classification as a transmembrane protein suggests potential involvement in:
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Membrane trafficking: Analogous to other Dictyostelium transmembrane proteins regulating endocytosis or macropinocytosis .
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Cell signaling: Possible interaction with conserved pathways like the γ-secretase complex, which modulates membrane dynamics in Dictyostelium .
Experimental Observations:
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Retention in membrane fractions: Observed in detergent-insoluble cytoskeletal preparations .
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Interaction assays: Direct binding partners remain unidentified, though yeast two-hybrid screens are pending .
Research Applications
Recombinant DDB_G0285385 is primarily used for:
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Antibody production: Immunogen for generating custom antibodies .
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Structural studies: Crystallization trials or cryo-EM to resolve 3D architecture.
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Pathway analysis: Screening for involvement in conserved signaling cascades (e.g., autophagy, phagocytosis) .
Outstanding Questions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order, and we will fulfill them accordingly.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance, as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: ddi:DDB_G0285385
