Recombinant Dictyostelium discoideum Putative ZDHHC-type palmitoyltransferase 4 (DDB_G0274251)
Description
Production and Purification
Recombinant DDB_G0274251 is commercially available as a lyophilized powder (>90% purity via SDS-PAGE) . Key production details include:
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Buffer Composition: Tris/PBS-based with 6% trehalose (pH 8.0) .
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Reconstitution: Recommended in sterile water with 50% glycerol for long-term stability .
Functional Annotations
Biological Roles
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Palmitoyltransferase Activity: Likely mediates S-palmitoylation of target proteins, influencing membrane localization and signaling .
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Immune Response: Homologs in other species (e.g., human ZDHHC4) regulate innate immunity and protein trafficking to the plasma membrane .
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Vesicle Trafficking: Indirectly linked to dynamin superfamily proteins in D. discoideum, which govern membrane dynamics and pathogen interactions .
Pathway Involvement
While specific pathways remain uncharacterized for DDB_G0274251, ZDHHC enzymes generally participate in:
Research Applications
Key Uses
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Antibody Development: Recombinant antibodies against D. discoideum antigens, including DDB_G0274251, enable precise protein localization and functional studies .
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Host-Pathogen Studies: D. discoideum DSPs (e.g., dynamin-like proteins) are models for analyzing bacterial infection mechanisms .
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Structural Studies: The protein’s conserved ZDHHC domain makes it a candidate for elucidating palmitoylation mechanisms .
Experimental Tools
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Proteomic Analysis: Detected in macropinosome proteomes, suggesting roles in endocytic trafficking .
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FRET-Based Sensors: Recombinant proteins facilitate dynamic studies of signaling molecules like c-di-GMP in D. discoideum .
Comparative Insights
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will fulfill your request to the best of our ability.
Note: Our proteins are standardly shipped with blue ice packs. If you require dry ice shipping, please communicate with us in advance as additional charges will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: ddi:DDB_G0274251
STRING: 44689.DDB0167795
