Recombinant Dinoroseobacter shibae Elongation factor Tu (tuf1)

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Cat. No.

BT32117

Source

Yeast

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32117

Source

E.coli

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32117

Source

E.coli

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32117

Source

Baculovirus

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32117

Source

Mammalian cell

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Purity

>85% (SDS-PAGE)

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Description

Molecular and Functional Overview

Elongation Factor Tu (EF-Tu) is a highly conserved GTPase responsible for delivering aminoacyl-tRNA to the ribosome during protein synthesis . In Dinoroseobacter shibae, EF-Tu is encoded by the tuf1 gene (UniProt ID: A8LQA8) . The recombinant form retains this canonical function but is engineered for experimental applications, including immunoblotting and enzyme-linked immunosorbent assays (ELISA) .

Research Applications

Pathogenesis Studies: EF-Tu’s role in bacterial adhesion and immune evasion could inform investigations into D. shibae's dual symbiotic-pathogenic behavior with algae .
Protein Interaction Mapping: Its recombinant form enables antibody generation for studying EF-Tu’s interaction with host ribosomes or extracellular matrices .
Biotechnological Tool: High-purity recombinant EF-Tu may serve as a standard in ribosomal translation assays or synthetic biology applications .

Knowledge Gaps and Future Directions

Current data lack direct studies on recombinant D. shibae EF-Tu’s moonlighting roles. Priority areas include:

Characterizing SLiM-mediated interactions in algal-bacterial symbiosis .
Assessing EF-Tu’s contribution to plasmid-driven pathogenicity (e.g., the "killer plasmid" pDS191) .
Structural resolution of D. shibae EF-Tu to compare with homologs like Escherichia coli EF-Tu .

Product Specs

Form

Lyophilized powder. We will ship the format in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchase method and location. Consult your local distributor for specifics. All proteins ship with normal blue ice packs. Request dry ice in advance (extra fees apply).

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type, please inform us, and we will prioritize its development.

Synonyms

tuf1; Dshi_0223;; tuf2; Dshi_0274Elongation factor Tu; EF-Tu

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-391

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

Target Names

tuf1

Target Protein Sequence

MAKEKFERSK PHVNIGTIGH VDHGKTTLTA AITKQFGDFK AYDEIDGAPE EKARGITIST AHVEYETDAR HYAHVDCPGH ADYVKNMITG AAQMDGAILV VNAADGPMPQ TREHILLGRQ VGIPYMVVFM NKVDQVDDEE LLELVEMEIR ELLSSYEYPG DDIPIIAGSA LAALEGRDPE IGEQKIAELM KAVDDYIPTP ARAVDQPFLM PIEDVFSISG RGTVVTGRVE RGVINVGDEI EIVGIRDTKK TTCTGVEMFR KLLDRGEAGD NIGALLRGVD REGVERGQVL CKPGSVTPHT KFEAEAYILT KEEGGRHTPF FANYRPQFYF RTTDVTGTVT LPEGTEMVMP GDNLKFGVEL IAPIAMEDGL RFAIREGGRT VGAGVVSKII E

Uniprot No.

A8LLG2

Target Background

Function

This protein promotes GTP-dependent binding of aminoacyl-tRNA to the ribosomal A-site during protein biosynthesis.

Database Links

KEGG: dsh:Dshi_0223

STRING: 398580.Dshi_0274

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily

Subcellular Location

Cytoplasm.

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Recombinant Dinoroseobacter shibae Elongation factor Tu (tuf1)

Description

Molecular and Functional Overview

Research Applications

Knowledge Gaps and Future Directions

Product Specs

Target Background

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