Recombinant Dinoroseobacter shibae Nucleoside diphosphate kinase (ndk)

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Cat. No.
BT54471
Source
Yeast
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54471
Source
E.coli
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54471
Source
E.coli
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54471
Source
Baculovirus
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54471
Source
Mammalian cell
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Purity
>85% (SDS-PAGE)
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Description

Molecular Characterization of Recombinant D. shibae NDK

Expression and purification:

  • Recombinant NDK is typically produced in Escherichia coli via plasmid vectors, followed by affinity chromatography .

  • Enzymatic activity assays (e.g., pyruvate kinase-lactate dehydrogenase coupled method) confirm phosphate transfer efficiency, with optimal activity at neutral pH and 25–37°C .

PropertyDetails
Molecular weight~16–18 kDa (based on homologs)
Catalytic residuesHis-117, Arg-104, Asp-120 (critical for phosphate transfer)
Optimal temperature33°C (matches D. shibae's growth optimum)
Substrate specificityBroad (ATP, GTP, CTP, UTP, dTDP)

Functional Role in D. shibae Metabolism

Metabolic integration:

  • NDK supplies GTP for dynamin-dependent membrane remodeling during cell division and vesicle internalization .

  • Supports denitrification pathways by maintaining nucleotide pools for reductases (e.g., nitrate reductase NapA, nitrite reductase NirS) .

Regulatory interactions:

  • Linked to the FnrL/Dnr regulatory network, which coordinates anaerobic respiration and stress responses .

  • Critical for resolving chromosomal dimers via XerCD recombinases at the dif site during cell division .

Research Applications and Findings

Biotechnological uses:

  • Enzyme kinetics: Studies on NDK homologs (e.g., A. flavus) reveal KM values of 0.2–0.5 mM for ATP and GTP, with inhibition by azidothymidine (AZT) .

  • DNA repair: D. shibae NDK assists in clearing overreplicated DNA via outer membrane vesicles (OMVs), preventing divisome obstruction .

Pathogen interaction:

  • The D. shibae NDK homolog in A. flavus regulates spore formation and virulence, suggesting analogous roles in algal symbiosis pathogenesis .

Comparative Analysis With Other NDKs

FeatureD. shibae NDKHuman NDK (Nm23)E. coli NDK
Subcellular localizationCytoplasmic, membrane-associated Cytoplasmic Cytoplasmic
Role in pathogenesisLinked to algal killing Tumor metastasis suppression Not reported
Structural motifHexameric Hexameric Hexameric

Future Directions

  • Industrial enzymes: Optimize NDK for nucleotide synthesis in bioreactors, leveraging D. shibae’s halotolerance .

  • Antimicrobial targets: Develop inhibitors targeting NDK’s active site (e.g., AZT derivatives) to disrupt bacterial-algal symbiosis .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but please specify any format requirements when ordering, and we will accommodate your request.
Lead Time
Delivery time varies depending on the purchase method and location. Please consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance, and additional fees will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, and the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing it.
Synonyms
ndk; Dshi_1350Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Nucleoside-2-P kinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-140
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)
Target Names
ndk
Target Protein Sequence
MAIERTLSII KPDATKRNLT GKINAKFEDA GLRIVAQKRI HLTKAQAGVF YAVHAERPFY DELCEFMASE PVVVQVLEGE GAIAKNREVM GATNPADAAP GTIRAEFAES VGENSVHGSD APETAAVEIA YFFSGLELVG
Uniprot No.
A8LIX8

Target Background

Function
Plays a major role in synthesizing nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism using a phosphorylated active-site intermediate.
Database Links

KEGG: dsh:Dshi_1350

STRING: 398580.Dshi_1350

Protein Families
NDK family
Subcellular Location
Cytoplasm.

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