Recombinant DNA-binding protein HU (hup)

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Cat. No.

BT39862

Source

Yeast

Synonyms

hup; hstHDNA-binding protein HU

Purity

>85% (SDS-PAGE)

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Cat. No.

BT39862

Source

E.coli

Synonyms

hup; hstHDNA-binding protein HU

Purity

>85% (SDS-PAGE)

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Cat. No.

BT39862

Source

E.coli

Synonyms

hup; hstHDNA-binding protein HU

Purity

>85% (SDS-PAGE)

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Cat. No.

BT39862

Source

Baculovirus

Synonyms

hup; hstHDNA-binding protein HU

Purity

>85% (SDS-PAGE)

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Cat. No.

BT39862

Source

Mammalian cell

Synonyms

hup; hstHDNA-binding protein HU

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant DNA-Binding Protein HU (hup)

Recombinant DNA-binding protein HU (hup) is a prokaryotic nucleoid-associated protein (NAP) critical for chromosomal architecture maintenance, DNA repair, and gene regulation. Produced via recombinant DNA technology, HU is conserved across bacterial species and functions as a structural and regulatory scaffold for nucleic acids . Unlike sequence-specific DNA-binding proteins, HU interacts with DNA through structural motifs rather than nucleotide sequences, enabling roles in DNA compaction, replication, recombination, and damage response .

2.1. Domain Architecture

HU exists as a dimer, with variations between species:

Escherichia coli: Heterodimer (HUαβ) encoded by hupA (α subunit) and hupB (β subunit) .
Mycobacterium tuberculosis: Homodimer (HUββ) encoded by hupB .
Helicobacter pylori: Homodimer (Hup) with conserved apical proline (P64) critical for DNA bending .

2.2. DNA Interaction Dynamics

HU binds DNA through two modules:

Invariable ds-DNA module: Recognized by HU’s β-ribbon arms .
Variable module: Contains ss-DNA or structural distortions (e.g., nicks, forks), contacted by the HU body .
This dual interaction confers 1,000-fold higher affinity for damaged DNA over intact ds-DNA .

3.1. DNA Repair and Recombination

Repair Intermediate Binding: HU stabilizes recombination intermediates (e.g., Holliday junctions, DNA overhangs) and protects DNA from exonucleases .
UV and γ-Irradiation Resistance: hup mutants exhibit hypersensitivity to UV and γ-rays due to impaired RecA-dependent repair pathways .
Abasic Site Repair: HU binds abasic (AP) sites in DNA, facilitating repair via base excision pathways .

Table 2: Key Functions of HU in DNA Dynamics

Function	Mechanism	Phenotype of hup Mutants
DNA Compaction	Induces negative supercoiling	Chromosome decompaction
Replication Initiation	Binds oriC to stabilize DnaA complexes	Delayed replication initiation
Transcriptional Regulation	Insulates transcription units via DNA bending	Altered gene expression

3.2. Role in Pathogenesis

Mycobacterium tuberculosis: HU is essential for DNA protection under oxidative stress, making it a potential drug target .
E. coli HU: Acetylation of Lys18 modulates interactions with host proteins like interleukin-1β during infection .

Table 3: Key Experimental Findings

Study Objective	Methodology	Key Result
HU-DNA binding specificity	EMSA, NMR	HU binds nicked DNA 1,000x tighter than ds-DNA
Role in UV resistance	Survival assays	hupAB mutants show 10x UV sensitivity
HU-RNA interaction	RNA electrophoretic shift	HU binds RNA-DNA hybrids > ds-DNA

4.2. Biotechnological Applications

DNA Nanotechnology: Recombinant HU stabilizes branched DNA structures for synthetic biology applications .
Antibiotic Target: Inhibitors targeting Mtb HU’s DNA-binding domain disrupt bacterial nucleoid organization .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins ship with standard blue ice packs. Contact us in advance for dry ice shipping (additional fees apply).

Notes

Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

hup; hstHDNA-binding protein HU

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-91

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Streptococcus thermophilus

Target Names

hup

Target Protein Sequence

MANKQDLIAK VAEATELTKK DSAAAVDAVF ASIEEFLAAG EKVQLIGFGN FEVRERAARQ GRNPQTGETI SIAASKVPAF KAGKALKDAV K

Uniprot No.

P96045

Target Background

Function

Histone-like DNA-binding protein HU (hup) stabilizes DNA by wrapping around it, preventing denaturation under extreme environmental conditions.

Protein Families

Bacterial histone-like protein family

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Recombinant DNA-binding protein HU (hup)

Description

Introduction to Recombinant DNA-Binding Protein HU (hup)

2.1. Domain Architecture

2.2. DNA Interaction Dynamics

3.1. DNA Repair and Recombination

Table 2: Key Functions of HU in DNA Dynamics

3.2. Role in Pathogenesis

Table 3: Key Experimental Findings

4.2. Biotechnological Applications

Product Specs

Target Background

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